The embryonic changes during the development of the freshwater prawn, Macrobrachium americanum are described from observations made on live embryos based on the percentage-staging method. Eggs were observed with a stereomicroscope to obtain descriptions of embryonic periods. This prawn has an incubation time of 18 days at 24◦C. Ten periods are described and illustrated. A comparison of this dev...

In order to explore the effects of probiotics on water quality, growth performance, nonspecific immunity, digestion, and intestinal flora Macrobrachium rosenbergii in biofloc culture system, three groups (six replicates each group) were set up divided into no bacteria (control group, Con), Bacillus subtilis (BS), effective microorganisms (EM) current experiment. After dissolution water, carbon ...

The gene encoding the capsid protein of Macrobrachium rosenbergii nodavirus (MrNV) was cloned into pGEX-6P-1 expression vector and then transformed into the Escherichia coli strain BL21. After induction, capsid protein-glutathione-S-transferase (GST-MrNV; 64 kDa) was produced. The recombinant protein was separated using SDS-PAGE, excised from the gel, electro-eluted and then used for immunizati...

In Crustacea, an early evolutionary group (∼50 000 species) inhabiting most ecological niches, sex differentiation is regulated by a male-specific androgenic gland (AG). The identification of AG-specific insulin-like factors (IAGs) and genomic sex markers offers an opportunity for a deeper understanding of the sexual differentiation mechanism in crustaceans and other arthropods. Here, we report...

Phylogenetic analysis of representatives from 18 wild populations of the giant freshwater prawn, Macrobrachium rosenbergii, utilising a fragment of the 16S rRNA mitochondrial gene, identified two major reciprocally monophyletic clades either side of a well-known biogeographic barrier, Huxley's line. The level of divergence between the two clades (maximum 6.2%) far exceeds divergence levels with...

Limitation of food availability (starvation) is known to influence the reproductive ability of animals. Autophagy is a lysosomal driven degradation process that protects the cell under metabolic stress conditions, such as during nutrient shortage. Whether, and how starvation-induced autophagy impacts on the maturation and function of reproductive organs in animals are still open questions. In t...

Macrobrachium tenellum and M. americanum are common freshwater prawns from the Americas' tropical subtropical Pacific continental side. They have different biological attributes currently threatened by human impact on their ecosystems. Both species provide quality products for consumption intensively exploited without proper management. This study analyzes, discusses, compares available informa...