Background: Malaria infection during pregnancy is a major public health problem in tropical and subtropical regions globally. Anaemia is often an adverse outcome of severe parasitic infections during pregnancy in developing countries. Pregnant women in malaria-endemic communities are more susceptible to Plasmodium falciparum infections than non-pregnant women of child-bearing age. Objective: To...

BACKGROUND To guide malaria elimination efforts in Swaziland and other countries, accurate assessments of transmission are critical. Pooled-PCR has potential to efficiently improve sensitivity to detect infections; serology may clarify temporal and spatial trends in exposure. METHODOLOGY/PRINCIPAL FINDINGS Using a stratified two-stage cluster, cross-sectional design, subjects were recruited f...

OBJECTIVE To check the epidemiology of malaria along Pak-Afghan bordering area.. METHODS The prospective study was conducted at Hatmi Medical Centre, Barmal district, Afghanistan, from March 2014 to February 2016, and comprised patients living in bordering areas between Pakistan's North Waziristan Agency and Afghanistan's Paktika province. Blood samples were collected from subjects with sympt...

The routine test for diagnosing malaria is still the thick blood smear, despite its known decreased sensitivity and specificity in situations of low parasite density and mixed infections. The polymerase chain reaction is increasingly being used for molecular detection and identification of Plasmodium species, due to its higher sensitivity and specificity. Nested PCR was performed on whole-blood...

BACKGROUND Malaria is endemic in Abbottabad and the adjoining areas like many other parts of the country. Depending upon the environmental conditions different species of malarial parasite are found in different areas. The present study was planned to see the pattern of malarial infection diagnosed at Ayub Teaching Hospital, Abbottabad. METHODS Giemsa stained thick and thin blood films of ind...

In spite of more than 30 years ofcontrol activities, malaria continues to be the most important parasitic infection in Malaysia, accounting for 39,189 confirmed cases in 1991, giving an annual parasite incidence rate of 2.2 per 1,000 population. Some factors contributing to the continued transmission of malaria are the development of drug resistant Plasmodium Jalciparum. changes in vector behav...

There is a critical need for developing new malaria diagnostic tools that are sensitive, cost effective and capable of performing large scale diagnosis. The real-time PCR methods are particularly robust for large scale screening and they can be used in malaria control and elimination programs. We have designed novel self-quenching photo-induced electron transfer (PET) fluorogenic primers for th...

INTRODUCTION Solomon Islands is intensifying national efforts to achieve malaria elimination. A long history of indoor spraying with residual insecticides, combined recently with distribution of long lasting insecticidal nets and artemether-lumefantrine therapy, has been implemented in Solomon Islands. The impact of these interventions on local endemicity of Plasmodium spp. is unknown. METHOD...

Malaria is an infectious disease caused by the bite of female Anopheles mosquitoes. There are four species, namely, Plasmodium vivax, Plasmodium falciparum, Plasmodium ovale and Plasmodium malariae causing human malaria. The difference between P.vivax malaria and P. falciparum malaria is that a person suffering from P. vivax infection can suffer relapses of the disease. The effect of a time del...

BACKGROUND Epidemiologic data on malaria are scant in many high-burden countries including the Democratic Republic of the Congo (DRC), which suffers the second-highest global burden of malaria. Malaria control efforts in regions with challenging infrastructure require reproducible and efficient surveillance. We employed new high-throughput molecular testing to characterize the state of malaria ...