Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

The mass profiles of cell-free extracts of 180 commensal and pathogenic strains of Escherichia coli were determined by MALDI-TOF mass spectrometry (MS). While some peaks were highly conserved in all E. coli, several peaks occurred only in some strains, showing heterogeneity among them. We did not detect strain-specific peaks for any of the E. coli categories tested. However, review of the fully...

This review compares and contrasts MALDI-MS, FT-IR spectroscopy Raman for whole organism fingerprinting bacterial typing.

Matrix Assisted Laser Desorption (MALDI) is a soft ionization technique used in modern day chemistry to analyze polymers with high precision and speed. This does not require any polymer standard for mass calibration (as the case of gel – permeation chromatography GPC). Protocols only minimum amounts solvent consumables. Measurements masses individual oligomers facilitate determination monomers ...

In the current issue of Clinical Chemistry, Braun et al. [1] describe a mutational analysis method that combines primer oligo base extension (PROBE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). This application further extends the use of MS for genetic analysis and suggests a possible role in the clinic. However, what is the long-term potentia...

Glycomics is a developing field that provides structural information on complex populations of glycans isolated from tissues, cells and organs. Strategies employing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) are central to glycomic analysis. Current MALDI-based glycomic strategies are capable of efficiently analyzing glycoprotein and glycosphingo...