in this study the effect of different concentrations of some abiotic elicitors (salicylic acid, jasmonic acid, cadmium chloride and sodium chloride) with different incubation times (3, 6, 9 and 12) on azadiractin production and cell growth in the cell culture of the iranian native neem were investigated. cell growth rate was measured by changes in dry cell weight and it was used as a basis for ...

introduction: laser phototherapy is used for the treatment of chemotherapy-induced oral mucositis in patients with leukemia, although there are limited data supporting the safety of this method. this study aimed to evaluate the effect of different doses of low-level laser on proliferation of acute myeloid leukemia (aml) cell line (kg-1a) in vitro. methods: a plastic flask containing 5,000,000 k...

aids (acquired immune deficiency syndrome), a result of human hiv (human immunodeficinency virus) infection, is one of the most troublesome world's health problems. extensive researches to find effective drugs for its treatment are running fast in huge capacities. imod (immuno-modulator drug) is the name of an herbal drug that has modulatory effects of immune system. as a goal of this research,...

improving the solubility of non-soluble herbal materials is an issue of interest in cell culture based experiments. evaluating the biological activity of these materials could become possible with the aid of cosolvents/surfactants which obviously should have little or no cytotoxic activity. in the present study, the cytotoxic activity of six cosolvents/surfactants: ethanol, methanol, tween 20 a...

objective: this study presents a simple method for isolation, expansion and purification of neonatal mouse spermatogonial stem cells. methods: we used enzymatic digestion to isolate a cell suspension of spermatogonia and sertoli cells from neonatal 2-day-old mice. the cells were cultured in dmem/f12 that contained 10% serum for two weeks. sertoli and spermatogonia cell characteristics were conf...

this study was designed to examine the effects of retinoic acid adding to cumulus and/or fibroblast cellsmonolayer on the development of mouse early embryos. one-cell mouse embryos were obtained from nmrimice after superovulation by an intraperitoneal injection of 5 iu equine chorionic gonadotrophin (ecg)followed 48 hrs later by 5 iu human chorionic gonadotrophin (hcg). mouse embryonic fibrobla...

s100ß a neurotrophic factor mainly released by astrocytes, has been implicated in the pathogenesis of bipolar disorder. thus, lithium may exert its neuroprotective effects to some extent through s100ß. furthermore, the possible effects of lithium on astrocytes as well as on interactions between neurons and astrocytes as a part of its mechanisms of actions are unknown. this study was undertaken...

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Cell culture media used in industrial mammalian cell culture are complex aqueous solutions that are inherently difficult to analyze comprehensively. The analysis of media quality and variance is of utmost importance in efficient manufacturing. We are exploring the use of rapid “holistic” analytical methods that can be used for routine screening of cell culture media used in industrial biotechno...