BACKGROUND Leptospirosis is an important but largely under-recognized public health problem in the tropics. Establishment of highly sensitive and specific laboratory diagnosis is essential to reveal the magnitude of problem and to improve treatment. This study aimed to evaluate the diagnostic accuracy of a recombinant LigA protein based IgM ELISA during outbreaks in the clinical-setting of a hi...

BACKGROUND Leptospirosis has a varied clinical presentation with complications like myocarditis and acute renal failure. There are many predictors of severity and mortality including clinical and laboratory parameters. Early detection and treatment can reduce complications. Therefore recognizing the early predictors of the complications of leptospirosis is important in patient management. This ...

s: Background & Objective: Leptospirosis is an emerging infectious disease, for the diagnosis of which clinical, epidemiological and laboratory parameters may be evaluated as per modidified Faine’s criteria suggested by several authors. The objective of this study was to validate the utility of modified Faine’s criteria in the diagnosis of leptospirosis. Methodology: This study was carried out ...

Leptospirosis is an endemic disease caused by Leptospira spp., a bacterium that affects animals and humans. In recent years, the number of reports leptospirosis in wild has increased, which highlights need to study infectious agents these animals. this study, duplex PCR for detection leptospiral DNA was performed on 50 kidney samples from bats, MAT (Microscopic Agglutination Test) serological a...

Two simple enzyme immunoassays, a conventional microplate and dot-ELISA, were developed to detect specific IgM antibodies using pool sonicated antigen prepared from three of the most reactive serovars of Leptospira associated with disease in Thailand. Both assays were evaluated and compared with the standard microscopic agglutination test (MAT) performed with 343 serum samples. A battery of 16 ...

Existing serological methods for diagnosis of leptospirosis are still unsatisfactorily due mainly to their low accuracy. In this study, serum samples of 18 clinically diagnosed-, IgM dipstick positive-, MAT positive-leptospirosis patients (group 1) were analyzed by IgG Western blotting against SDS-PAGE separated-whole cell homogenates of pathogenic and non-pathogenic Leptospira spp. belonging t...

the importance of cats in the leptospira epidemiology is due to the possibility of transferring leptospirosis to wild and domesticated animals. the purpose of this survey was to determine the prevalence of leptospira infection in shorthair cats in different location of mashhad, iran. totally, 147 blood samples were taken from 42 (28.57%), 52 (35.37%) and 53 (36.05%) households, stray and cats w...

Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sampl...

BACKGROUND Leptospirosis is an important disease caused by various serovars of Leptospira sp. It can affect humans as well as domestic and wild animals; therefore, it has importance for public health, animal production, and wild species. The aim of this paper is to discuss the epidemiology of animal leptospirosis in Rio de Janeiro, Brazil, as a possible model for other tropical regions. In seve...

AbstractsObjective This is the first study was conducted to identify Leptospirosis seroprevalence and serovar distribution among healthy people in different regions Vietnam. Methods cross-sectional with 600 asymptomatic three provinces, representing for North, northern Central, South of Vietnam from November December 2019. Each participant collected a 3 ml venous bloo...