نتایج جستجو برای: mdbk
تعداد نتایج: 281 فیلتر نتایج به سال:
BACKGROUND: Infectious Bovine Rhinotrachitis (IBR) caus-ed by Bovine Herpes Virus-1 (BoHV-1) was first, observed in one of the Tehran Industrial cattle breeders, with importation of cattle in 1965. Later, the isolation of bovine herpes virus approved the existence of the disease in the most of the provinces of the Iran. OBJECTIVES: Presence of antibodies against BoHV-1 was evaluated in young bo...
cancers belong to a group of disorders which are very important for researchers. because they have several types and cause mortality in human beings. many investigations are performing in order to introduce cheaper drugs with lower side effects especially with natural sources. ferula genus grows all over the world but some of them are endemic to iran. many investigations have proved differe...
Understanding the pathogenic mechanisms of Mycobacterium avium subspecies paratuberculosis (MAP) and the host responses to Johne's disease is complicated by the multi-faceted disease progression, late-onset host reaction and the lack of available ex vivo infection models. We describe a novel cell culture passage model that mimics the course of infection in vivo. The developed model simulates th...
Bovine herpesvirus 1 (BoHV-1) is widely distributed among cattle populations and has been associated with cells, fluids, and tissues collected from donor animals for use in reproductive technologies. The purpose of this study was to determine if lactoferrin would inhibit BoHV-1 in cell culture and to evaluate if embryos could develop normally when cultured in vitro with lactoferrin. In Experime...
Johne's disease, caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a severe chronic enteritis which affects large populations of ruminants globally. Prevention strategies to combat the spread of Johne's disease among cattle herds involve adhering to strict calving practices to ensure young susceptible animals do not come in contact with MAP-contaminated colostrum, milk, or fec...
The action of a number of toxins used in the formation of immunotoxins was studied in polarized cells. Diphtheria toxin inhibited protein synthesis most efficiently when added to the basolateral side of the kidney cells, MDCK-I, MDBK and Pt K2, and the colon carcinoma cell Caco-2. Similar findings were made with Pseudomonas aeruginosa exotoxin A in MDCK-I, Pt K2, and Caco-2 cells, and with mode...
Using real-time RT-PCR and Western blot analysis in bovine kidney epithelial cells, we systematically investigated the effects of butyrate on patterns of gene expression relevant to DNA replication apparatus. The real-time PCR and Western blot data generally confirmed previously reported microarray data. Of the five genes tested by quantitative RT-PCR, CDKN1A (p21(waf1)) was up regulated, CDC2/...
The carbohydrate structure of the BIN and F glycoproteins of the paramyxovirus SVS grown in bovine kidney (MDBK) cells has been elucidated by newly developed micromethods. The glycoproteins were purified by chromatography on fetuin coupled to Sepharose and on hydroxylapatite. Extensive aggregation of the F glycoprotein at concentrations > 0.1 mg/ml was reduced by carboxymethylation. The glycopo...
Neonatal döneminde en önemli mortalite sebebi olan buzağı ishalleri birçok faktöre bağlı olarak gelişmekte ve büyük ekonomik kayıplara sebep olmaktadır. Türkiye’ de bugüne kadar yapılan epidemiyolojik çalışmalarda, viral etkenlerin yeni doğan buzağılarda oranda neonatal ishal olgularına neden olduğu gösterilmiştir. Bu çalışmada şiddetli ölümlerin görüldüğü çoklu enfeksiyonların rolü araştırıldı...
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