BACKGROUND In 2016 the World Health Organization declared the mosquito-borne Zika virus (ZIKV) a "public health emergency of international concern." ZIKV is a blood-borne pathogen, which therefore causes concerns regarding the safety of human plasma-derived products due to potential contamination of the blood supply. This study investigated the effectiveness of viral inactivation steps used dur...

Ultraviolet disinfection is an important method for controlling the large-scale outbreaks of diseases in aquaculture. As a novel and promising light source, ultraviolet light-emitting diode (UV-LED) has advantages safety, high efficiency no environmental pollution risks. However, it remains unclear whether UV-LEDs can replace traditional UV sources aquaculture water treatment processes. Present...

GATA4 and GATA6 are central cardiac transcriptional regulators. The postnatal, stage-specific function of the cardiac transcription factors GATA4 and GATA6 have not been evaluated. In part, this is because current Cre-loxP approaches to cardiac gene inactivation require time consuming and costly breeding of Cre-expressing and "floxed" mouse lines, often with limited control of the extent or tim...

UV inactivation, photoreactivation, and dark repair of Escherichia coli and Cryptosporidium parvum were investigated with the endonuclease sensitive site (ESS) assay, which can determine UV-induced pyrimidine dimers in the genomic DNA of microorganisms. In a 99.9% inactivation of E. coli, high correlation was observed between the dose of UV irradiation and the number of pyrimidine dimers induce...

Abstract Enteric bacteria such as Escherichia coli and Salmonella spp. are significant fish pathogens related to thousands of cases food-borne diseases every year in human. Since aquatic environments reservoirs these pathogens, they may contaminate the food result outbreaks. Therefore, it is crucial reduce or eliminate from aquaculture facilities. We tested effectiveness three commonly used che...

Ultraviolet (UV) radiation treatment is an effective method for purifying pollutant water contaminated with bacteria and/or chemicals. As emerging technology, purification by deep ultraviolet light-emitting diodes (DUV-LEDs) promising. Few studies have used the point-source characteristics of LEDs and instead replaced mercury vapor lamps LEDs. Here, we show our recent progress in instantaneous ...

Radiation inactivation of the red beet (Beta vulgaris L.) plasma membrane ATPase was carried out using -y-ray radiation from a '37Cs source. Inactivation of vanadate-sensitive ATPase activity by y-ray radiation followed an exponential decline with increasing total dose, indicating a single target size calculated to have a molecular weight of about 228,000. Since the catalytic subunit of the red...

Single ventricular myocytes of adult mice were prepared by enzymatic dissociation for voltage clamp experiments with the one suction pipette dialysis method. After blocking the Na current by 10(-4) mol/l TTX early outward currents (IEO) with incomplete inactivation could be elicited by clamping from -50 mV to test potentials (VT) positive to -30 mV. Interfering Ca currents were very small (less...

Incubation of isolated rat hepatocytes with oxytocin produces a time- and dose-dependent inactivation of glycogen synthase. Such inactivation is associated with an increase in the phosphorylation state of the 88 kDa subunit of the enzyme, as observed after electrophoretic analysis of the 32P-labelled enzyme isolated by immunoprecipitation from cells incubated with [32P]phosphate. CNBr cleavage ...