ABSTRACT In mammals, sperm-borne regulators can be transferred to oocytes during fertilization and have different effects on the formation of pronuclei, first cleavage zygotes, development preimplantation embryos even metabolism individuals after birth. The regulatory role sperm microRNAs (miRNAs) in bovine has not been reported detail. By constructing screening miRNA expression libraries, we f...

In order to study the bacterial flora of dead-in-shell ostrich chicks, twelve unhatched eggs which did nothave external pipping during the hatching period were transferred to the laboratory of microbiology. The eggshells were accurately disinfected and the embryos were removed and placed in a sterile plate. The surface of each embryo was swabbed with a sterile swab which was also plunged throug...

The aim of this study was to evaluate the effect of sex determination procedures in Day 7 to Day 8 bovine embryos of various quality. For the purposes of comparison we used high quality (HQ) as well as lower quality (LQ) embryos obtained from superovulated donors. The healthy embryonic cells isolated from HQ embryos and blastomeres protruding into the perivitelline space of LQ embryos, were ana...

Co-culture of human embryos (n = 384 cycles) to the blastocyst stage using Vero cell monolayers was carried out between August 1995 and December 1997. A total of 2868 zygotes were co-cultured and 1027 embryos reached the blastocyst stage (blastocyst formation rate 35.8%). The blastocysts were frozen in 43.7% of patients. A mean of 1.8 blastocysts was transferred per patient and 95 pregnancies w...

Background: Morphology and presence of Polar body in the oocyte are denoting oocyte quality and is used as a criterion for prognosis of embryo quality. However, little is known about ICSI result of oocytes with two polar bodies. The present study reports the result of ICSI in a case having several oocytes with two polar bodies. Materials and Methods: A 34 years old patient (K.H.) with history o...

Background One of the important complications of assisted reproductive technologies is multiple pregnancy, the aim of the present study was to indentify the risk factors of multiple pregnancy independent of number of transfred embryos. MaterialsAndMethods This retrospective study reviewed the medical records of patients underwent intracytoplasmic sperm injection (ICSI) cycle in Royan Institute ...

Background With aging, the ovarian reserve is decreased and that is a major contributor to poor ovarian response to exogenous gonadotropins. The aim of the present study is to evaluate the role of Dexamethasone on ovarian response in infertile patients aged over 35 years undergoing IVF/ICSI cycles. MaterialsAndMethods In this triple blind placbo-control clinical trial study, a total of 72 infer...

BACKGROUND With infertility populations rapidly aging, treatments improving pregnancy chances assume increasing clinical importance. Dehydroepiandrosterone (DHEA) has been reported to improve pregnancy rates and lower miscarriage rates in women with diminished ovarian function. This study was planned to evaluate whether pretreatment with DHEA may improve in vitro fertilization (IVF) parameters ...