We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity screening in a bacterial selection system. We characterized the substrate specificity and kinetics of these endonucleases by monitoring DNA cleavage events with deep sequencing. The endonuclease specificities revea...

abstract background: accumulation of secretions in the airways of patients with an endotracheal tube and mechanical ventilation will have serious consequences. one of the most common methods of airway clearance is endotracheal suctioning. in order to facilitate discharge of airway secretion resulting in promotion of gas exchange, chest physiotherapy techniques can be used at the time of expirat...

An important factor in material quality is the time duration that materials are used (known as substance lifetime). Lifetime is a function of several factors, and among them wearing and abrasion resistances are more important than the other aspects. In addition, to the physical and mechanical properties, abrasion has a significant effect on the textiles appearance. This phenomenon considerably ...

Due to their widespread availability, sandstones are commonly used as a natural stone for buildings, monuments and sculptures. In thecurrent study, nine sandstone samples from the Upper Red Formation (specified by the letters A, B, C, CG, S, S1, Sh, Tr and Min)were studied from a petrophysical point of view and their durability was evaluated. Then, their suitability as building stones wasassess...

Mechanical stimulation can prevent adipogenic and improve osteogenic lineage allocation of mesenchymal stem cells (MSC), an effect associated with the preservation of -catenin levels. We asked whether mechanical up-regulation of -catenin was critical to reduction in adipogenesis as well as other mechanical events inducing alternate MSC lineage selection. In MSC cultured under strong adipogenic ...

OBJECTIVES All-suture anchors are increasingly used in rotator cuff repair procedures. Potential benefits include decreased bone damage. However, there is limited published evidence for the relative strength of fixation for all-suture anchors compared with traditional anchors. MATERIALS AND METHODS A total of four commercially available all-suture anchors, the 'Y-Knot' (ConMed), Q-FIX (Smith ...

parameters in-situ during the transformation and to obtain SUPERELASTICITY is a deformation mechanism phase-specific information. However, neutrons penetrate observed in NiTi and other allotropic, ordered alloys where deeper than X-rays (several millimeters, compared to a few the high-temperature cubic phase (austenite), when subjected micrometers for X-rays from a conventional source). Thus, t...

BACKGROUND Mechanical torque limiting devices (MTLDs) are necessary tools to control a peak torque and achieving target values of screw component of dental implants. Due to probable effect of autoclaving and number of use on the accuracy of these devices, this study aimed to evaluate the effect of sterilization and number of use on the accuracy of friction-style mechanical torque limiting devic...

BACKGROUND Atrial fibrillation (AF) is the most common clinically significant cardiac arrhythmia. However, diagnosis of intraventricular dyssynchrony in patients with AF is difficult due to beat-to-beat variation. Additionally, evaluation of mechanical dyssynchrony in the traditional method is based on average of 5 to 10 beats, which is exhausting and time consuming. Single-beat evaluation of a...

friction welding is widely used in various industries. in friction welding, heat is generated by conversion of mechanical energy into thermal energy at the interface the work pieces during pin rotation under pressure. a three-dimensional thermo mechanical simulation of friction stir welding (fsw) processes is carried out for aluminium alloys of 6061and 7050 where the simulation results are comp...