A method is presented for isolation of 2-10 gm of highly puri fied nucleoli from Walker tumors. Initially, 0.5-1 kg of tumor was minced in a specially devised stainless steel mincer. The minced tissue was then homogenized in a continuous tissue homogenizer in 0.25 Msucrose containing 3.3 HIMCaClj. The nuclear preparation, obtained by continuous flow centrifugation, was subjected to sonic oscill...

A comparison has been made of the methods used for homogenizing and processing tissues in hydrocarbon-skin protein binding studies. The procedure of choice is to use a glass homogenizer cooled in ice and to precipitate the proteins with acetone. The zerotime binding of a number of hydrocarbons has been studied and has been found to be negligible. The zero-time binding of several derivatives of ...

This article reports the development and evaluation of two nano-emulsions (F45T-03/HFB and F15T-03/PFOB) containing fluorinated trityl radicals dissolved in perfluorocarbons. Preparation with a high-pressure homogenizer conferred sub-micronic size to both nano-emulsions. In vitro and in vivo EPR spectroscopy showed that the nano-emulsions had much greater oxygen sensitivity than the hydrophilic...

This paper described a fast, simple, and small scale method of sample preparation followed by High-Performance Liquid Chromatography (HPLC) coupled Photo-Diode Array (PDA) detector for quantification of canthaxanthin in chicken liver and fat, and hen’s egg yolk. The HPLC-PDA was performed on a C18 column with an isocratic mobile phase. Analyte was extracted from the sample using a handheld ultr...

UNLABELLED HOMO.pl is a perl script that allows extracting important registers from an extensive data table or microarrays results. It is very useful in data mining for microarrays analysis. HOMO works as a homogenizer that converts the initial data table into more specific and manageable data according to a list of important genes or terms. This is very useful when a pathway, a condition, or a...

introduction: cholestasis is impairment of normal bile excretion into the duodenum and classified as mechanical and non mechanical cholestasis. mechanical cholestasis presents with increase in bile duct diameter or obstruction in bile duct in an ercp. cholestasis leads to different complications. one of these complications is mucosal peptic erosion leading to gastrointestinal bleeding, perforat...

Biodegradable poly(D, L-lactide-co-glycolide) (PLGA) and PLGA-based polymeric nanoparticles are widely used for sustained release of protein and peptide drugs. These formulations are usually prepared by water/oil/water (W/O/W) and solid/oil/water (S/O/W) double emulsion solvent evaporation method. Other methods of preparation are nanoprecipitation, emulsion solvent diffusion and salting-out. Th...

A simple and rapid multi-residue method was developed for the determination of 28 kinds of veterinary drugs and feed additives (drugs) in muscle of cattle, pig and chicken. The drugs were extracted with acetonitrile-water (95:5) in a homogenizer and ultrasonic generator. The extracted solution was poured into an alumina column and the drugs were eluted with acetonitrile-water (90:10). The eluat...

A reliable extraction method is described for the preparation of total nucleic acids from at least ten plant genera for subsequent detection of plant pathogens by PCR-based techniques. The method combined a modified CTAB (cetyltrimethylammonium bromide) extraction protocol with a semi-automatic homogenizer (FastPrep) instrument) for rapid sample processing and low potential of cross contaminati...