Treatments wilh the herbicides chlorpropham (isopropyl m-chlorocarbinilate), DCPA (dimethyl tetrachloroterephthalate), and El'TC (S-ethyl dipropylthiocarbamate), alone or in combination with Meloidogyne hapla Chilwood, significantly reduced the growth of both nemalode-resistant 'Nev Syn XX' atttt susceptible 'Ranger" alfalfa (Medicago saliva L.) seedlings. M. hapla infection of both alfalfas wa...

The establishment of a nitrogen-fixing symbiosis is an economically important plant phenomenon. Biological reduction of dinitrogen to ammonia is among the most effective fixation systems facilitating the plant growth in nitrogen starved soils without the requirement of massive inputs of fertilizers. This process occurs in legumes roots in structures called nodules. Nodule development is induced...

An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leav...

The composition of phosphorus-containing compounds of intact lyophilized alfalfa tissue has been determined, in part, by solid-state (31)P nuclear magnetic resonance. The tissue (Medicago sativa L., cv Regen S; and some of its crosses) was grown in culture under both nonregenerating and regenerating conditions, the latter enhanced by the addition of specific amino acids. Analysis of the (31)P n...

In order to obtain a salt-tolerant perennial alfalfa (Medicago sativa L.), we transferred the halophyte Salicornia europaea L. Na(+)/H(+) antiporter gene, SeNHX1, to alfalfa by using the Agrobacterium-mediated transformation method. The transformants were confirmed by both PCR and RT-PCR analyses. Of 197 plants that were obtained after transformation, 36 were positive by PCR analysis using 2 pr...

In the next decades, forage legumes are likely to become more important. However, predicted climate change may increase the risk of droughts and thus influence their agricultural performance. Decreases in yield due to water shortage are well documented, while influences on nutritive values are inconsistent. Therefore, we examined the effects of drought on crude protein (CP), neutral deterge...