Ionically crosslinked alginate hydrogels are attractive scaffolds because of their biocompatibility and mild gelation reaction that allows for gentle cell incorporation. However, the instability of ionically crosslinked hydrogels in an aqueous environment is a challenge that limits their application. This report presents a novel method to control the dimensions and mechanical properties of ioni...

Temperature-sensitive, unsaturated fatty acid (fabB) auxotrophs of Escherichia coli can grow at the restrictive temperature in the absence of unsaturated fatty acid in a medium with a high osmotic pressure. If a mutant culture was starved for unsaturated fatty acids and harvested just before the lysis started, the fatty acid composition of the cells was the same as that of cells grown until lat...

BACKGROUND: Lovastatin is known to inhibit its own synthesis in the fungus Aspergillus terreus. Therefore, the use of a fermentation strategy that continuously removes some of the lovastatin produced from the bioreactor can enhance its productivity. This paper reports on the effects of dilution rate and the composition of the feed medium on lovastatin production by A. terreus. RESULTS: The feed...

The interspecific hybrid fir Abies cilicica × Abies cephalonica is based on native species Abies cephalonica (Loud.) and Abies cilicica (Ant. et Kotschy) Carr. Many variants of nutrient media have been tested. The medium BAP at a concentration of 1 mg·l–1, 1,000 mg·l–1 myo-inositol, 500 mg·l–1 glutamine, and 1,000 mg·l–1 casein was found to be the most suitable initiation medium for somatic emb...

Electropermeabilization and cell death caused by the exposure to high voltage electric pulses depends on the parameters of pulses, as well as the composition of the extracellular medium. We studied the influence of extracellular conductivity on electropermeabilization and survival of cells in vitro. For this purpose, we used a physiological medium with a conductivity of 1.6 S/m and three artifi...

The role of human capital composition has been given importance in the most recent endogenous growth models. Assuming that primary as well as secondary education is more suitable for imitation and higher education is more appropriate for innovation, this paper empirically investigates whether the contribution of human capital to productivity growth depends on the composition of human capital an...

Rodent organotypic hippocampal cultures spontaneously develop epileptiform activity after approximately 2 weeks in vitro and are increasingly used as a model of chronic post-traumatic epilepsy. However, organotypic cultures are maintained in an artificial environment (culture medium), which contains electrolytes, glucose, amino acids and other components that are not present at the same concent...

The effect of the ionic environment of the incubating medium upon glycogen formation by liver slices was first reported by Ostern, Herbert, and Holmes (1). Using rabbit liver slices, they found that, aerobically, calcium stimulated glycogen formation in a modified Ringer’s medium containing 1 per cent glucose. Addition of phosphate to this medium resuhed in a decrease in glycogen synthesis. The...