Techniques have been developed for the isolation of basal bodies with cilia attached or for the isolation of only basal bodies from the rabbit oviduct. Oviducts are removed, cut open, and placed in an extraction medium composed of 0.25 M sucrose, 0.001 M EDTA, 0.025 M KCl, 0.02 M Hepes buffer pH 7.5, and 0.05% Triton X-100. After the oviduct is agitated in this medium on a Vortex mixer for (1/2...

The object of the investigation is to study reducible $M$-ideals in Banach spaces. It is shown that if the number of $M$-ideals in a Banach space $X$ is $n(<infty)$, then the number of reducible $M$-ideals does not exceed of $frac{(n-2)(n-3)}{2}$. Moreover, given a compact metric space $X$, we obtain a general form of a reducible $M$-ideal in the space $C(X)$ of continuous functions on $X$. The...

Mycobacterium avium subsp. paratuberculosis is shed into the milk and feces of cows with advanced Johne's disease, allowing the transmission of M. avium subsp. paratuberculosis between animals. The objective of this study was to formulate an optimized protocol for the isolation of M. avium subsp. paratuberculosis in milk. The parameters investigated included chemical decontamination with N-acet...

The addition of D-glucose, D-galactose, or D-mannose to culture medium increased survival of heated Chinese hamster ovary cells in a concentration- and time-dependent manner. Heat protection by sugars was not immediate but required prior incubation in the sugar medium before hyperthermia. The degree of heat protection conferred by each sugar and its time dependence differed characteristically: ...

Generally zooplankton growth is often limited by the quality of their algal diet. A cheaper common practice in aquaculture, is to culture algae with fertilizers; however, the demography of zooplankton when fed these algae has not yet been evaluated. We studied the population growth and life table demography of the rotifers Anuraeopsis fissa and Brachionus rubens, and the cladoceran Moina macroc...

Cancer stem cells (CSCs) are the sole population possessing high self-renewal activity in tumors, with their existence affecting tumor recurrence. However, the invasive activity of CSCs has yet to be fully understood. In this article, we established a tumor sphere culture of RSV-M mouse glioma cells (RSV-M-TS) and evaluated their migration and invasion activities. Histological analysis of a tum...

We study the effect of homogenization on flame propagation in periodic excitable media, when the width of the flame is much smaller than the characteristic size of the heterogeneities.

To establish an efficient regeneration system for Anthurium andreanum cv Rubrun, seeds from plant spadixes were germinated on a medium supplemented with 2.2 μ M BA. After 2 weeks, 74% of the seeds germinated and four weeks later, micro-cuttings from these plantlets were subcultured on a medium containing 4.4 μ M BA and 0.05 μ M NAA. On average, 3.6 shoots per explant were obtained. Four weeks o...

A two-step procedure was devised for purification of alpha 2-macroglobulin (alpha 2 M) from human serum by high performance liquid chromatography (HPLC) on "Superose 6" gel-filtration medium and "Mono-Q" ion-exchange medium. The isolated protein reacts with specific alpha 2 M-anti-serum upon immunoelectrophoresis, migrates as single protein band on SDS-acrylamide gel electrophoresis, and yields...