The effects of substrates and inhibitors on the conformation of yeast cytoplasmic pyruvate decarboxylase (Z-oxo-acid carboxy-lyase, EC 4 .l .l .l) has been examined using the technique of automated tritium-hydrogen exchange. This experimental approach, as used in this study, reflects primarily the number of solvent-inaccessible or “core” hydrogens in the protein. Yeast cytoplasmic pyruvate deca...

1. A method is described for the estimation of thiol ester groups. The thiol ester is converted into the corresponding thiol by reaction with ammonia; the thiol is then titrated amperometrically with mercuric chloride. 2. The method may be used in the presence of SH and S.S groups. The SH groups are titrated at pH3 in the presence of excess of chloride; under these conditions thiol esters do no...

A method is presented for the rapid determination of total mercury in blood. The reagent used is alkaline sodium borohydride, and no digestion of the sample is needed. The detection limit and sensitivity are 1.15 and 1.59 ng of Hg, respectively. The method gives reproducible results (the CV ranged from 5.3 to 6.7% for low and high mercury blood samples, respectively), comparable to those obtain...

Salinity and heavy metals affect the growth and development of higher plants and also their productivity. In the present study, Cauliflower (Brassica oleracea var botrytis) seeds were allowed to germinate and grow in the absence (control) and presence of different concentrations (50, 100, 250, 500, 750 and 1000 μM) of NaCl, CdCl2, HgCl2 and ZnCl2. Seed germination and growth parameters of seedl...

Mercury chloride is a common heavy metal found in the environment, and it endangers both environment living organisms. The study aimed to show whether Flavonoids Fractions of Adansonia digitata (FAD) could protect rats from HgCl2-induced hepatorena toxicity. Thirty (30) were randomly assigned one six groups. first group was given no HgCl2 as control, while second single daily dose (0.5 mg/kg). ...

Mercury chloride (HgCl2) is widely used in industries; fact, this chemical substance deadly for health and causes various problems, such as liver damage, kidney hematopoietic disorders, especially erythropoiesis. This study aims to determine the blood profile Rattus nurvegicus exposed HgCl2 by combining IR Bagendit rice leaves-infused water young coconut water. employed an experimental method e...