In Arabidopsis, the root meristem originates from the hypophyseal cell and from an adjoining cell tier that is distinct at the heart stage of embryogenesis. We have analysed mutations in the HOBBIT (HBT) gene that is essential for root meristem formation. hbt embryos display incorrect hypophyseal cell development from the quadrant stage onward. At the heart stage, the adjoining cell tier of hbt...

There are presented the results of a comparative study in an experimental culture central agro-climatic zone Belarus, influence method vegetative propagation plants (cuttings and microclonal) on antioxidant enzymatic (catalase, peroxidase polyphenol oxidase)activity fruits early ripening (Weymouth) late (Elizabeth Atlantik) varieties V. corymbosum L. It was shown that against background signifi...

Abstract Plant cells cultivated in vitro are a convenient model for studying the genetic and physiological mechanisms necessary to acquire state of pluripotency. Earlier studies on plant Arabidopsis thaliana (L.) Heynh. have identified key role genes that determine pluripotency shoot apical meristem de novo regeneration tissue culture. In accordance with this, mutant plants higher level express...

Aim. The aim of the study was obtaining Camelina sativa (L.) Crantz lines with yeast genes trehalose synthesis TPS1 and TPS2 to increase their resistance drought. Methods. Seeds C. genotype FEORZhYaF-1 were used for in vitro culture establishment. For this hypocotyl segments shoot meristems 5-days-old camelina seedlings cultivated on three different nutrient media regeneration supplemented vari...

Shoot-borne crown roots are the major root system in cereals. Previous work has shown that the Wuschel-related homeobox gene WOX11 is necessary and sufficient to promote rice (Oryza sativa) crown root emergence and elongation. Here, we show that WOX11 recruits the ADA2-GCN5 histone acetyltransferase module to activate downstream target genes in crown root meristem. Rice ADA2 and GCN5 genes are ...

We have investigated the effects on plant development of mutations in the Arabidopsis thaliana CLAVATA1 gene. In clavata1 plants, vegetative, inflorescence and floral meristems are all enlarged relative to wild type. The apical meristem can fasciate in the more severe mutant alleles, and this fasciation can occur prior to the transition to flowering. Flowers of clavata1 plants can have increase...

To maintain proper meristem function, cell division and differentiation must be coordinately regulated in distinct subdomains of the meristem. Although a number of regulators necessary for the correct organization of the shoot apical meristem (SAM) have been identified, it is still largely unknown how their function is integrated with the cell cycle machinery to translate domain identity into c...

The first step in flower development is the generation of a floral meristem by the inflorescence meristem. We have analyzed how this process is affected by mutant alleles of the Arabidopsis gene LEAFY. We show that LEAFY interacts with another floral control gene, APETALA1, to promote the transition from inflorescence to floral meristem. We have cloned the LEAFY gene, and, consistent with the m...

The root meristem consists of populations of distal and proximal stem cells and an organizing center known as the quiescent center. During embryogenesis, initiation of the root meristem occurs when an asymmetric cell division of the hypophysis forms the distal stem cells and quiescent center. We have identified NO TRANSMITTING TRACT (NTT) and two closely related paralogs as being required for t...

Meristems require a myriad of intercellular signaling pathways for coordination of cell division within and between functional zones and clonal cell layers. This control of cell division ensures a constant availability of stem cells throughout the life span of the meristem while limiting overproliferation of meristematic cells and maintaining the meristem structure. We have undertaken a genetic...