A recombinant Factor VIII (Factor VIII-delta II) consists of a unique polypeptide chain of 165 kDa deleted from the major part of the B-domain and from the cleavage site at Arg-1648-Glu-1649 found in plasma-derived Factor VIII. It was expressed in mammalian cells in serum-free medium containing von Willebrand factor and purified by a one-step immunopurification. The recombinant Factor VIII was ...

This work studies the use of biological and combined biological/enzymatic treatments in phenol degradation. The systems studied were conventional batch aerobic biological followed or preceded by enzymatic treatment. Tyrosinase extracted from the mushroom Agaricus bispora was employed. Biological treatment efficiently degraded effluents containing up to 420 mg.L of phenol, removing 97% of the CO...

Factor VIII coagulant activity (VIII(C)) and the von Willebrand's disease antigen (Factor VIII-like antigen, vW-Ag) are biologically linked, and it has been suggested that they reside on the same molecule. However, insolubilized human isoantibody to VIII(C) and rabbit antiserum containing antibodies to VIII(C) and vW-Ag differentially bind and remove these entities from plasma, thus physically ...

Coagulants exert a significant influence on removing turbidity, TSS and COD. This study has examined the effects of poly-aluminium chloride (PAC), aluminium sulphate (alum) and ferric chloride on removal of turbidity, TSS, COD from greywater in residential complexes of miners working in decorative stone mines. Also, a comparison was undertaken with the electrocoagulation system to find out whet...

Plantago ovata is a medicinal plant of plantain, Plantaginaceae, family used to treat intestinal disorders and stomach diseases. P. ovata grain has relieving effects and healing inflammation and irritation of the mucous layer of stomach and duodenal ulcer. Beside the medicinal benefits, this plant is useful for water turbidity removal. Coagulation and flocculation are important processes in wat...

Antibodies against factor VIII coagulant activity can appear in haemophiliacs who are treated with factor VIII preparations but also spontaneously in non-haemophiliacs. The Bethesda assay is the most commonly used method to detect these antibodies, but it lacks specificity especially in the lower range resulting in unreliable data. Two modifications are proposed and tested to resolve the imperf...

The natural water contamination because of the population growth, industrialization, urbanization, sewage from households, industries, institutions, and hospitals, among other things, has increased. After using in various ways, this turns into wastewater, completing hydrological cycle. Waterways that are contaminated pose numerous hazards to health environment. As a result, contaminants must be...