This study was performed to assess the neurotoxic effects of methylmercury, arsanilic acid and danofloxacin by quantification of neural-specific proteins in vitro. Quantitation of the protein markers during 14 days of differentiation indicated that the mouse ESCs were completely differentiated into neural cells by Day 8. The cells were treated with non-cytotoxic concentrations of three chemical...

Methylmercury (MeHg) is an environmental contaminant that can have adverse effects on wildlife. Because MeHg is produced by bacteria in aquatic ecosystems, studies of MeHg contamination of food webs historically have focused on aquatic organisms. However, recent studies have shown that terrestrial organisms such as songbirds can be contaminated with MeHg by feeding on MeHg-contaminated spiders....

Nationally, the most important source of exposure to any mercury compound is the consumption of fish. Certain populations may have occupational exposures and in certain areas of NJ consumption of water from private wells can be a significant source of mercury exposure (see Volume II Chapter 7). The extent of exposure to mercury from cultural uses is not known and such practices appear to be lim...

Worldwide, people’s diets are their main source of exposure to most persistent organic pollutants (POPs).1,2 Fish is a major contributor to POP intake and also the primary dietary source for methylmercury. A new statistical modeling tool discussed in EHP suggests that fish consumption advisories are unlikely to effectively reduce prenatal exposures to polychlorinated biphenyls (PCBs) and other ...

Mercury is found in the environment as elemental mercury, inorganic mercury or organic mercury (ethyl-, methyl-, alkyl-, or phenylmercury). In our environment it is present in thermometers, thermostats, dental amalgams, paint, cosmetic products, laxatives, diuretics and antiseptics. Organic mercury is the most toxic and most frequent form of mercury exposure, found in fish, poultry, pesticides ...

Selenium (Se) supplementation in the nutritionally relevant range counteracts methylmercury (MeHg) toxicity. Since Se tends to be abundant in fish, MeHg exposures alone may not provide an accurate index of risk from fish consumption. Molar ratios of MeHg:Se in the diets and Hg:Se in tissues of exposed individuals may provide a more accurate index. This experiment compared MeHg toxicity in relat...

We describe a novel genetic screen that is performed by transfecting every individual clone of an expression clone collection into a separate population of cells in a high-throughput mode. We combined high-throughput functional genomics with experimental validation to discover human genes that ameliorate cytotoxic responses of neuronal HT-22 cells upon exposure to oxidative stress. A collection...