The basic building block of a gene regulatory network consists of a gene encoding a transcription factor (TF) and the gene(s) it regulates. Considerable efforts have been directed recently at devising experiments and algorithms to determine TFs and their corresponding target genes using gene expression and other types of data. The underlying problem is that the expression of a gene coding for t...

Kinetic rate constants for enzymatic reactions are typically measured with a series of experiments at different substrate concentrations in a well-mixed container. Here we demonstrate a microfluidic technique for measuring Michaelis-Menten rate constants with only a single experiment. Enzyme and substrate are brought together in a coflow microfluidic device, and we establish analytically and nu...

The oxidation of alcohols, n-propanol, 2-methoxy ethanol and 2-ethoxy by cerium (IV) in nitric acid leads to the formation corresponding aldehydes. reaction is first order with respect Ce(IV). A Michaelis-Menten type kinetics observed alcohols. retarded initial [Ce(IV)] increase concentration oxidant. exhibits participation 2 mole 1 It has been concluded that Ce(IV)-dimer participate process fr...

It is shown here that a suitable form for estimation and inference using the Michaelis-Menten [(1913) Biochem Z. 49, 333-369] model for simple enzymic reactions is one in which the two parameters appear in the denominator of the equation. In this form, convergence to the least-squares estimates using the Gauss-Newton method [see Kennedy & Gentle (1980) Statistical Computing, Marcel Dekker, New ...

Starting from the detailed catalytic mechanism of a biocatalyst we provide a coarse-graining procedure which, by construction, is thermodynamically consistent. Œis procedure provides stoichiometries, reaction ƒuxes (rate laws), and reaction forces (Gibbs energies of reaction) for the coarse-grained level. It can treat active transporters and molecular machines, and thus extends the applicabilit...

Abbreviated expressions for enzyme kinetic expressions, such as the Michaelis-Menten (M-M) equations, are based on the premise that enzyme concentrations are low compared with those of the substrate and product. When one does progress experiments, where the solute is consumed during conversion to form a series of products, the idealized conditions are violated. Here, we analyzed data of xanthin...