Zebrafish have long been utilized to study the cellular and molecular mechanisms of development by time-lapse imaging of the living transparent embryo. Here we describe a method to mount zebrafish embryos for long-term imaging and demonstrate how to automate the capture of time-lapse images using a confocal microscope. We also describe a method to create controlled, precise damage to individual...

Toru Hyodo, MD, PhD, Department of Urology and Anatomy, Faculty of Medicine, Tottori University, 36-1 Nishi-Machi, Yonago 683 (Japan) Dear Sir, Birch and Fairley [1, 2] and Birch et al. [3] described glomerular bleeding giving rise to a wide range of morphological alterations in red cells, including dysmorphic red cells. They also reported that phase contrast microscopy was clinically useful fo...

The method of preparation of bronchoalveolar lavage fluid (BALF) for cytological examination can significantly affect the results of cellular quantitation. Investigations have shown that cytocentrifugation leads to an underestimation of the number of lymphocytes and membrane filter preparation to an underestimation of the number of neutrophils. As a simple alternative to these two techniques, B...

Some issues are considered which arise when edge detection is used to identify cells in diierential interference contrast (DIC) microscope images. A method for outlining the cells in such images is developed and a simple practical example illustrated. Edge detection is complex due to the nature of DIC images, in which an apparent light eeect is captured across the image. Further complications a...

zeolite fau type-y is one of the most studied framework of all zeolites, and has been used as catalysts for number of reactions in the refinery and petrochemical industry. in this research, nanocrystalline zeolite y was synthesized by hydrothermal method. the crystal size of zeolite y is influenced by temperature, aging time, alkalinity, and water content. the synthesized zeolite nay is identif...

background and objective: glumerular diseases are among the most prevalent causes of renal chronic insufficiencies. this study aimed to analyze the prevalence of various kinds of glumerulonephritis based on the findings of electron microscope. materials and methods: this study had a descriptive retrospective and cross sectional strategy. slides of patients (124 cases) who had undergone kidney b...