objective(s): to understand the role of mitochondrial respiration in cisplatin sensitivity, we have employed wild-type and mitochondrial dna depleted rho0 yeast cells. materials and methods: wild type and rho0 yeast cultured in fermentable and non-fermentable sugar containing media, were studied for their sensitivity against cisplatin by monitoring growth curves, oxygen consumption, ph changes ...

Mitochondrial Cytochrome Oxidase Subunit I DNA sequences are a good discriminative marker for phylogenetic studies in crustaceans and especially in amphipoda. In the present study, molecular and morphological data were analyzed to test whether Gammarus lobifer authority and Gammarus balutchi authority which one or two geographically separated but morphologically similar species. The analyses pr...

Research on ancient DNA (aDNA) has the potential to enable molecular biologists and archeologists to decipher certain aspects of history by direct looking into the past. However, several major problems in this field limit the applicability of aDNA studies, most importantly contamination with modern DNA and postmortem DNA degradation. In this study we extracted and analyzed aDNA obtained from ~3...

The molecular diagnosis of mitochondrial disorders still remains elusive in a large proportion of patients, but advances in next generation sequencing are significantly improving our chances to detect mutations even in sporadic patients. Syndromes associated with mitochondrial DNA multiple deletions are caused by different molecular defects resulting in a wide spectrum of predominantly adult-on...

Context Species classification disputes can be resolved using integrative taxonomy, which involves the use of both phenotypic and genetic information to determine species boundaries. Aims Our aim was clarify boundaries two commercially important cryptic halfbeak (Hemiramphidae), whose distributions overlap in south-eastern Australia, assist fisheries management. Methods We applied an taxonomic ...

The analysis of mitochondrial DNA (mtDNA) hypervariable region (HVR) sequence data from ancient human remains provides valuable insights into the genetic structure and population dynamics populations. mtDNA is particularly useful in studying populations, because it maternally inherited has a higher mutation rate compared to nuclear DNA. To determine three Colombian pre-Hispanic populations comp...