Two assays were developed to quantitate the adherence of an Escherichia coli strain (RDEC-1) known to colonize the mucosal surface of the small intestine of rabbits to brush borders isolated from rabbit intestinal epithelial cells. In the first assay, the mean adherence per rabbit brush border was determined by counting the number of organisms adhering to each of 40 brush borders under phase mi...

Technologies ranging from solvent extraction and drug delivery to tissue engineering are beginning to benefit from the unique ability of “smart polymers” to undergo controllable structural changes in response to external stimuli. The prototype is poly(N-isopropylacrylamide) (P(NIPAAm)) which exhibits an abrupt and reversible hydrophilic to hydrophobic transition above its lower critical solutio...

Conductive transport of chloride ion is important in controlling ion and fluid secretion by exocrine tissues. The current study was directed at identifying proteins in the intestinal brush-border membrane that may be involved with conductive chloride transport. Reaction of total brush-border membrane protein with phenyl-isothiocyanate inhibited conductive chloride transport into brush-border me...

Computerized oral brush biopsy became commercially available in 1999. The objectives of this study were to provide senior dental students with the clinical competency to perform oral brush biopsy and to evaluate the extent to which students used this technique and the outcomes of their biopsy techniques. During the 2004-05 academic year, 114 senior dental students at The Ohio State University C...

Purified brush border and basolateral membranes were isolated from homogenized intestinal enterocytes of Holstein steers by divalent cation precipitation followed by differential and sucrose density gradient centrifugation. Alkaline phosphatase and Na/K adenosine triphosphatase served as marker enzymes for the brush border and basolateral membranes, respectively. The brush border and basolatera...

The complex interaction of polyelectrolyte multilayers (PEMs) physisorbed onto end-grafted polymer brushes with focus on the temperature-responsive behavior of the system is addressed in this work. The investigated brush/multilayer composite consists of a poly(styrene sulfonate)/poly(diallyldimethylammonium chloride) (PSS/PDADMAC) multilayer deposited onto the poly(N-isopropylacrylamide-b-dimet...

The pale-headed brush-finch (Atlapetes pallidiceps) is threatened with extinction due to habitat loss, but very little is known about its ecological requirements. We used multiple logistic regression to study habitat selection of this species at landscape, territory, and nest site scales in order to make recommendations about effective management. Habitat selection by the sympatric stripe-heade...

Statement of problem: Spatial-resolved near-infrared spectroscopy enables non-invasive continuous measurement of " tissue oxygenation index " (TOI), which correlates to mixed venous oxygenation (SvO2). The aim of the present study was to compare cerebral TOI and peripheral TOI. Statement of methods: Repeated simultaneous measurements on right forehead and calf, were performed in 10 neonates wit...