Serology, defined as antibody-based diagnostics, has been regarded as the diagnostic gold standard in transfusion medicine. Nowadays however the impact of molecular diagnostics in transfusion medicine is rapidly growing. Molecular diagnostics can improve tissue typing (HLA typing), increase safety of blood products (NAT testing of infectious diseases), and enable blood group typing in difficult...

Since its emergence, semiconductor nanoparticles known as quantum dots (QDs) have drawn considerable attention and have quickly extended their applicability to numerous fields within the life sciences. This is largely due to their unique optical properties such as high brightness and narrow emission band as well as other advantages over traditional organic fluorophores. New molecular sensing st...

The major advances made over the past few years in molecular and cell biology are providing a progressively more detailed understanding of the molecular pathways that control normal processes and become dysregulated in disease [1]. This has resulted in the documentation of numerous genetic, epigenetic, transcriptomic, proteomic and metabolomic biomarkers that promise earlier disease detection, ...

Human Papilloma Virus (HPV) is becoming a menace worldwide, especially to the developing world, due to its involvement in a variety of malignancies, with cervical cancer being the most important and prevalent. There are many HPV types; HPV 16/18 are the most carcinogenic but few others are also characterized as high-risk (HR). They can cause a variety of low- or high-grade cellular abnormalitie...

BACKGROUND The Ca(2+)-dependent protein phosphatase enzyme calcineurin (Cn) (protein phosphatase 3) is best known for its role as director of the adaptive immune response. One of its principal substrates is the nuclear factor of activated T cells (NFAT), which translocates to the nucleus after dephosphorylation to mediate gene transcription. Drugs targeting Cn (the Cn inhibitors tacrolimus and ...

Assay Manufacturer Method Detection limit or dynamic range (IU/mL) Qualitative HCV RNA dection assays AmplicorTM HCV 2.0 Roche Molecular System Manual RT-PCR 50 Cobas AmplicorTM HCV 2.0 Roche Molecular System Semi-automated RT-PCR 50 VersantTM HCV Siemens Health Care Diagnostics Semi-automated TMA 10 Quantitative HCV RNA detection assays AmplicorTM HCV Monitor Roche Molecular System Manual RT-P...

EDITORIAL article Front. Mol. Biosci., 28 July 2023Sec. Molecular Diagnostics and Therapeutics Volume 10 - 2023 | https://doi.org/10.3389/fmolb.2023.1248534

Analysis from Maternal Plasma and Nucleated Blood Cells, Maddalena Smid, Fiorenza Lagona, Lara de Benassuti, Augusto Ferrari, Maurizio Ferrari, and Laura Cremonesi (Istituto di Ricovero e Cura a Carattere Scientifico, Hospital San Raffaele, 1 Department of Obstetrics and Gynecology and 2 Unit of Genetics and Molecular Diagnostics, Via Olgettina 60, 20132 Milan, Italy; * address correspondence t...

Molecular diagnostics (MDx) is currently a clinical reality that has its roots deep in the study of gene function, structure, and regulation. The multitude of human mutations identified in the various genetic diseases can now be assayed in the clinical molecular diagnostics laboratory. The polymerase chain reaction (PCR) has facilitated the transition from the research to the clinical laborator...

Semiconductor quantum dots (QDs) are a new class of fluorophores with unique physical and chemical properties, which allow to appreciably expand the possibilities for the current methods of fluorescent imaging and optical diagnostics. Here we discuss the prospects of QD application for molecular diagnostics of tumors ranging from cancer-specific marker detection on microplates to non-invasive t...