genetic diversity among plants sprouted from seven year old clones of mulberry (morus indica var. s36) was studied using molecular markers such as issr and rapd. the clones showed significant variability in sex expression and the sprouting behavior. these changes were appeared after seven years of rigorous pruning and training (pruned once in every 3-4months). genetic diversity analysis reveale...

Angelica gigas Nakai is an important medicinal herb, widely utilized in Asian countries especially in Korea, Japan, and China. Although it is a vital medicinal herb, the lack of sequencing data and efficient molecular markers has limited the application of a genetic approach for horticultural improvements. Simple sequence repeats (SSRs) are universally accepted molecular markers for population ...

staphylococcus aureus carrying pvl gene remain major health problem associated with highly virulent infections. characterization of such gene is important to know the impact and the functional significance of nucleotide variations. pcr and standard sequencing were performed for twelve sudanese strains from different sources. protein structures prediction, modeling and physiochemical analysis we...

The cultivated apple (Malus domestica) is one of the most important fruit crops cultivated in different regions of the world including Iran. For production and breeding of high quality apple, the knowledge of genetic diversity of the cultivated apples  is  necessary. Therefore we studied genetic diversity of 25 genotypes by using ISSR molecular markers. In present study, ten ISSR primers produc...

Background: Male infertility has many causes, including genetic infertility. The NOP2/Sun domain family, member7 (Nsun7) gene, which encodes putative methyltransferase Nsun7, has a role in sperm motility. The aim of the present study was to investigate the effect of the T26248G polymorphism on Nsun7 protein function and its role in male infertility. Materials and Methods: Semen samples were col...

The genus Cinnamomum of the family Lauraceae is an economically important crop. Ceylon cinnamon (Cinnamomum verum syn. C. zeylanicum) has exceptional position in global market. In addition to cultivated species, Sri Lanka home seven endemic wild capparu-coronde, citriodorum, dubium, litseifolium, ovalifolium, rivulorum, and sinharajaense. Nevertheless, species delimitation not been successful w...

Orphan crops, which include many of the tropical fruit species used in juice industry, lack genomic resources and breeding efforts. Typical this dilemma is commercial cultivars purple passion fruit, Passiflora edulis f. edulis, information on genetic its substantial semiwild gene pool. In study, we develop single-nucleotide polymorphism (SNP) markers for show that diversity crop has been reduce...

in india, holstein and its crosses are being used extensively in breeding programmes and all these breeding bulls are screened for autosomal recessive genes. blood samples are collected in ethylenediaminetetraacetic acid (edta) coated tubes and dna was isolated by using phenol-chloroform method. polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) wereperformed by using...

1. Abstract 2. Introduction 3. Genotyping methods 3.1. Genetic markers 3.1.1. Insertion sequences 3.1.2. Short repetitive DNA sequences 3.2. Restriction fragment length polymorphism analysis 3.2.1. IS6110 RFLP analysis 3.2.2. PGRS RFLP analysis 3.3. PCR-based typing 3.3.1. Mixed-linker PCR 3.3.2. Spoligotyping 3.3.3. VNTR and MIRU-VNTR typing 3.4. Comparison of genotyping methods 4. Molecular e...