Canine distemper virus (CDV) causes a life-threatening disease in several carnivores including domestic dogs. Recently, we identified a molecule, CD9, a member of the tetraspan transmembrane protein family, which facilitates, and antibodies to which inhibit, the infection of tissue culture cells with CDV (strain Onderstepoort). Here we describe that an anti-CD9 monoclonal antibody (MAb K41) did...

UNLABELLED A human monoclonal heterosubtypic antibody, MAb 3.1, with its heavy chain encoded by VH3-30, was isolated using phage display with immobilized hemagglutinin (HA) from influenza virus A/Japan/305/1957(H2N2) as the target. Antibody 3.1 potently neutralizes influenza viruses from the H1a clade (i.e., H1, H2, H5, H6) but has little neutralizing activity against the H1b clade. Its crystal...

Protein biologics now represent a signifi cant share of pharmaceutical research and development efforts. Their specifi city has been their key to success, as their cell-specifi c modality and relative lack of compound-specifi c toxicity have provided safer and more effective means to treat several chronic diseases. The most common class of proteins used as pharmaceuticals is monoclonal antibodi...

BACKGROUND Monoclonal antibodies are a major class of biological therapies in human medicine but have not yet been successfully applied to veterinary species. We have developed a novel approach, PETisation, to rapidly convert antibodies for use in veterinary species. As an example, anti-nerve growth factor (anti-NGF) monoclonal antibodies (mAbs) which are effective in reducing acute and chronic...

Therapeutic monoclonal antibodies (mAbs) are dominating the biopharmaceutical field due to fact of their high specificity in treatment diverse diseases. Nevertheless, mAbs very complex glycoproteins exhibiting several macro- and microheterogeneities that may affect safety, quality, efficacy. This complexity is challenging for development, formulation, quality control. To tackle issue, a combina...

Abstract Rationale: Reduction of mast cells (MCs) through stem cell factor (SCF) neutralization combined with inhibition the alarmin TSLP in a single molecule may result broader efficacy inflammatory disorders. Methods: Monoclonal antibodies (mAb) separately targeting SCF and were generated from immunized mice. A potent neutralizing antibody against each target was selected humanized. Bispecifi...

background : production of monoclonal antibodies to leishmania antigens assists the identification and characterization of these organisms. objective: production of monoclonal antibodies against epitopes on the gp63. methods: two murine monoclonal antibodies to gp63 were produced and characterized. the reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

Monoclonal antibody (MAb) 667 is a neutralizing mouse monoclonal antibody recognizing the envelope glycoprotein (Env) of the ecotropic neurotropic murine retrovirus CasBrE but not that of other murine retroviruses. Since 667 can be used for preclinical studies of antiviral gene therapy as well as for studying the early events of retroviral infection, we have cloned its cDNAs and molecularly cha...

226 words) 23 Vertical HIV-1 transmission via breastfeeding is the predominant contributor to pediatric 24 infections that are ongoing in this era of highly effective antiretroviral therapy (ART). 25 Remarkably, only ~10% of infants chronically exposed to the virus via breastfeeding from 26 untreated HIV-infected mothers become infected, suggesting the presence of naturally protective 27 factor...

The envelope protein (E1-E2) of Hepatitis C virus (HCV) is a major component of the viral structure. The glycosylated envelope protein is considered to be important for initiation of infection by binding to cellular receptor(s) and also known as one of the major antigenic targets to host immune response. The present study was aimed at identifying mouse monoclonal antibodies which inhibit bindin...