1. The aim of this study was to determine whether seed crystals of uric acid or monosodium urate promote the epitaxial deposition of calcium oxalate in undiluted human urine. The effects of seed crystals of uric acid, monosodium urate or calcium oxalate on calcium oxalate crystallization induced in pooled 24-h urine samples collected from six healthy men were determined by [14C]oxalate depositi...

PC12, a clonal line of rat pheochromocytoma, accumulates newly synthesized acetylcholine in storage granules. The accumulation of acetylcholine in PC12 granules, but not acetylcholine synthesis, was inhibited by treatment of the cells with any of several inhibitors of energy metabolism. These included nigericin, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, dicyclohexylcarbodiimide, and i...

Cytotoxic agents, cadmium, iodoacetate, thio-TEPA, podophyllin, colchicine, and alcohol, were instilled in one uterine horn of the rat to observe their subsequent eflect on fertility. Cadmium and iodoacetate interfere with implantation in both horns without altering ovulation for periods of 1 to 2 months. Thio-TEPA produces a unilateral efiect for periods of 2 months. With alcohol this unilater...

NOELL (1952, 1953a, b, 1955) reported that sodium iodoacetate (NaIA), administered intravenously to rabbits, selectively destroyed the visual cells of the retina, an effect he attributed to interference with glycolysis. In previous work (Sorsby, Newhouse, and Lucas, 1957), we investigated the effects on the retina of a wide range of thiol reagents, administered intravenously at maximum tolerate...

Incubation of rat adipose-tissue microsomal fractions with iodoacetate caused an inactivation of glycerol phosphate acyltransferase that could be prevented by the presence of palmitoyl-CoA. A microsomal protein of subunit Mr 54 000 was found to react with radioactively labelled iodoacetate in the absence, but not in the presence, of palmitoyl-CoA. It is suggested that this protein is a componen...

Pig heart isocitrate dehydrogenase, which catalyzes the TPN-dependent dehydrogenation of isocitrate as well as the decarboxylation of oxalosuccinate, is inactivated by treatment with iodoacetate at pH 5.5 and 30’. Approximately 1 mole of “C-labeled reagent is incorporated per mole of protein. The effect of alkylation on the isocitrate dehydrogenase activity is greater than that on the oxalosucc...

Alterations produced by iodoacetate in visual cells have been studied under the electron microscope. Lesions of the outer segments of the rods are visible as early as 3 hours after a single injection of 20 mg. iodoacetate per kg. body weight. After 6 hours the changes are more marked and consist then of disorganization, vesiculation, and lysis of the rod sacs. The inner segments of most rod cel...