Multipotent adult progenitor cells (MAPCs) are bone marrow-derived stem cells that have extensive in vitro expansion capacity and can differentiate in vivo and in vitro into tissue cells of all 3 germinal layers: ectoderm, mesoderm, and endoderm. The origin of MAPCs within bone marrow is unknown. MAPCs are believed to be derived from the bone marrow stroma compartment as they are isolated withi...

objective(s):sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. this study was performed   to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rbmmsc) into osteogenic lineage. materials and methods: isolated rbmmsc were grown in dmem supplemented with 10% fbs. the cells were exposed to different concen...

background: langerhans' cell histiocytosis (lch) is a reactive proliferative disease of unknown pathogenesis characterized by proliferation of langerhans cells. involvement of bone marrow (bm), liver and lung are related to high risk factors and poor survival. the aim of this report is to highlight the clinical and haematological findings of 5 cases of lch with bm infiltration which may help to...

Although nucleated red blood cells (nRBCs) are rarely found circulating in older children, they are commonly seen in the blood of newborns. They are primarily produced in the fetal bone marrow in response to erythropoietin and are stored in the marrow as precursors to reticulocytes and mature erythrocytes. Many acute and chronic stimuli cause increases in the number of circulating nRBCs from ei...

Aplastic anemia is an abnormal immune reaction disease in which T lymphocytes destroy hematopoietic stem and progenitor cells because of immune hyperactivity. Bone marrow mesenchymal stem cells (BMSCs) have hematopoietic supporting and immune regulation functions. This study investigated BMSCs homing in mice transplantation models after bone marrow failure. BALB/c mice were randomly divided int...

Reciprocal bone marrow transfers were performed between mouse strains sensitive or resistant to 3-methylcholanthrene-induced thymic lymphoma. Sensitivity and resistance are properties inherent in bone marrow, and cannot be altered by maturation of marrow in an environment of the opposite phenotype.

Normal mouse serum (NMS) devoid of colony-stimulating factor (CSF) was found to enhance the interleukin 3 (IL-3)-driven colony formation of bone marrow in vitro. Inclusion of NMS in bone marrow colony-forming assays resulted in greatly increased numbers of colonies and clusters following seven days incubation; however, incubation of bone marrow with NMS before the colony-forming assay had no ef...

We investigated whether the stem cell that reconstitutes total erythropoiesis of a WBB6F1-W/Wv mouse differentiates into lymphoid lineage. The electrophoretic pattern of hemoglobin was used as a marker of the reconstitution; 3-phosphoglycerate kinase (PGK), an X chromosome-linked enzyme was used as a tool for estimating clonality. We injected 10(5) bone marrow cells of 5-FU treated C57BL/6-Pgk-...

The expression and function of a receptor tyrosine kinase, c-kit, in the adult bone marrow of the mouse were investigated by using monoclonal antibodies (mAbs) against the extracellular domain of murine c-kit. In adult C57BL/6 mouse, 7.8% of total bone marrow cells express c-kit on their surface. Half of the c-kit+ cells do not express lineage markers including Mac-1, Gr-1, TER-119, and B220, w...