Nowadays, three dimensional (3D) cell cultures are widely used in the biological laboratories and several optical clearing approaches have been proposed to visualize individual cells deepest layers of cancer multicellular spheroids. However, defining most appropriate approach for different lines is an open issue due lack a gold standard quantitative metric. In this article, we describe share si...

Mesenchymal stem cells (MSCs) have been previously explored as a part of cell-based therapies for the repair of damaged cartilage. Current MSC chondrogenic differentiation strategies employ large pellets; however, we have developed a technique to form small MSC aggregates (500–1,000 cells) that can reduce transport barriers while maintaining a multicellular structure analogous to cartilaginous ...