AIMS To optimise a one step reverse transcriptase polymerase chain reaction (RT-PCR) protocol for BCR-ABL chimaera detection. METHODS Compared with published RT-PCR procedures, this novel approach has at least two advantages. First, the same enzyme is used for both reverse transcription and PCR. Second, amplification of the target (BCR-ABL chimaera) and control gene (ABL) is performed simulta...

Dengue is a mosquito borne disease caused by any of the four serotypes of dengue virus. Dengue 4 virus (DEN-4) was once the predominant serotype found in Malaysia causing up to 60% of dengue fever (DF) cases in 1960'sl. Since then, however, other serotypes notably DEN-2 and DEN-3 have become the predominant serotypes isolated among DF and dengue hemorrhagic fever (DHF) patients. DEN-4 made up o...

Background and Aim: Infectious bursal disease (IBD) is an infectious immunosuppressive that affects young chickens. Instead of strict biosecurity practices, vaccination used to control IBD. However, the has not been effectively managed. Variations in observed clinical symptoms lead confounding diagnoses. The study aimed obtain pathological lesion data from chickens suspected IBD virus (IBDV) in...

One-step reverse-transcription quantitative polymerase chain reaction (qRT-PCR) is the most widely applied method for COVID-19 diagnostics. Notwithstanding facts that one-step qRT-PCR well suited diagnosis of and there are many commercially available kits in market, their high cost unavailability due to airport closures shipment restriction became a major bottleneck had driven desire produce ke...

A reverse transcriptase (RT)-nested-polymerase chain reaction (PCR) was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related (CER) cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emph...

Background: Dengue fever is a mosquito-borne disease which is not known to be endemic in Iran. Case Report: In October 2015, a 32-year-old Iranian woman was admitted with acute unexplained high-grade fever, headache, pain, rash, diarrhea, leukopenia and elevated liver enzymes after returning from India. Serological and molecular analysis for Dengue virus (DENV) infection revealed positive ...

We developed reverse transcriptase (RT) PCR assays for the detection of mRNA from three spliced genes of human herpesvirus 6 (HHV-6), the immediate-early genes U16/U17 and U89/U90 and the late gene U60/U66. Sequence analysis determined the splicing sites of these genes. The new assays may be instrumental in investigating the association between HHV-6 and disease.

Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex TaqMan or real-time semiquantitative rever...