objective: mycoplasma salivarium (m. salivarium) isone of the most common contaminants present in cell culture laboratories that cause undesirable effects on cell cultures. thus, the identification and rapid diagnosis in controlling and prevention of this contaminant are important. the aim of this study is the detection of mycoplasma salivarium contamination in cell culture using polymerase cha...

bacterial vaginosis or non-specific vaginitis describes the disease caused by a change in the normal flora of the vagina, which leads to the elimination of lactobacilli, generating hydrogen peroxide and excess growth of bacteria, particularly anaerobic bacteria. this disease is the most prevalent infection of the female genital tract, and the rate of frequency of anaerobic bacteria, specificall...

Mycoplasma agalactia was cultured in PPLO broth medium supplemented with sterilized normal horse serum in fermentor. Cultivation process continued at 37 ºC under aerobic conditions and growth was terminated after 26 hours cultured period with 2.2×109 Mycoplasma per ml. Samples were taken at different stages of growth. Culture was inactivated with 0.04 %v/v formaldehyde and the vaccine was adjuv...

Abstract Contamination of primary and cell cultures by mycoplasmas is one the main economic biological pitfalls in basic research, diagnosis manufacture biotechnological products. It a common issue which may be difficult to conduct surveillance on. Mycoplasma presence affect several physiological parameters cell, besides being considered an important source inaccurate and/or non-reproducible sc...

Almost complete (> 96%) 16S rRNA sequences from nine ruminant mycoplasmas have been determined by solid-phase DNA sequencing. Polymorphisms were found in four of the 16S rRNA sequences, which indicated the existence of two different rRNA operons. Seven polymorphisms were found in Mycoplasma agalatiae, three were found in Mycoplasma bovis, one was found in Mycoplasma alkalescens, and one was fou...

Mycoplasma spp. are a major cause of mastitis, arthritis and pneumonia in cattle, and have been associated with reproductive disorders in cows. While culture is the traditional method of identification the use of PCR has become more common. Several investigators have developed PCR protocols to detect M. bovis in milk, yet few studies have evaluated other sample types or other important Mycoplas...