Cryopreservation features of Lithuanian garlic (Allium sativum L.) cultivars ‘Žiemiai’ and ‘Dangiai’ were investigated. The survival rate state explants, depending on the type explant, dehydration conditions evaluated in experiment. Explants isolated from cloves bulbils dehydrated with a plant vitrification solution 3 (PVS 3) containing 50% w/v glycerol sucrose liquid MS medium. Three different...

To accelerate breeding of Pinus massoniana Lamb. resistance to pine wilt disease (PWD), caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus, a protocol was established for in vitro propagation P. and evaluation regenerated microshoots from different clones PWN. Axillary bud induction achieved culturing cotyledonary node explants 3-week-old seedlings Gupta Durzan (DCR) medium that ...

A complete protocol is presented for in vitro regeneration of pomegranate (Punica granatum L.), a tropical fruit tree, using cotyledonary nodes derived from axenic seedlings. Shoot development was induced from cotyledonary nodes on Murashige and Skoog (1962) (MS) medium supplemented with 2.3±23.0 mM benzyladenine (BA) or kinetin (Kn). Both type and concentration of cytokinin signi®cantly in ̄uen...

Broussonetia papyrifera is an important ecological and economic tree species. The sexual reproduction of B. not only has a low germination rate, but also requires high environmental conditions. Therefore, asexual propagation using tissue culture can effectively improve the efficiency papyrifera. In this study, leaves budded shoots were used as explants, different concentrations plant growth reg...

Embryogenic calli were induced from longitudinally bisected segments of protocorm-like bodies (PLBs) of Cymbidium Twilight Moon ‘Day Light’, a hybrid orchid, on modified Vacin and Went medium [Bot. Gaz. 110 (1949) 605] supplemented with 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) within 1 mont...

The tightly regulated expression patterns of structural cell wall proteins in several plant species indicate that they play a crucial role in determining the extracellular matrix structure for specific cell types. We demonstrate that AtPRP3, a proline-rich cell wall protein in Arabidopsis, is expressed in root-hair-bearing epidermal cells at the root/shoot junction and within the root different...

Phenotypical alterations observed in rolB-transformed plants have been proposed to result from a rise in intracellular free auxin due to a RolB-catalyzed hydrolysis of auxin conjugates (J.J. Estruch, J. Schell, A. Spena [1991] EMBO J 10: 3125-3128). We have investigated this hypothesis in detail using tobacco (Nicofiana tabacum) mesophyll protoplasts isolated from plants transformed with the ro...

fig trees are threatened by the attack of fig mosaic disease (fmd) on leaves and fruits caused by viruses of several genera. shoot-tip culture is a convenient method for viral sanitation. for this purpose, a reliable protocol for rapid in vitro propagation was developed with shoot-tips of three major tunisian local fig (ficus carica l.) varieties zidi (zdi), soltani (sni), bither abiadh (ba) an...

Cremastra appendiculata var. variabilis (Blume) I.D. Lund), also known as single-leaf cremastra (SLC), is a rare and threatened species native to Korea, it has the potential be grown beautiful flowering pot or garden plant. There still no reliable strategy multiply SLC. Thus, an effective method for propagating was needed its conservation mass production. In present study, we examined effects o...

Aim of study: (i) to explore differences between eggplant flowers capable setting fruit including long (LGs) and medium style (MEs) those which suffer from severe problems with fertility short ones (SRTs); (ii) study the effect plant growth regulators on floral morphology setting.
 Area Isfahan University Technology, Isfahan, Iran, 2017 2018.
 Material methods: First initial 13 genoty...