background: boophilus tick is responsible for the transmission of some pathogens (types of germs) and the reduction of fertility in cattles. tropomyosin protein plays an important role in regulating actin function, immune and allergic reactions and vaccine production. objectives: accurate identification of antigenic determinants (epitopes) is difficult, time consuming and not economic, thus we ...

In the present study, we investigated whether activation of protease-activated receptor type 2 (PAR-2) with SLIGRL (SL)NH2, a short mimetic agonistic peptide, directly stimulates pepsinogen secretion from gastric-isolated, pepsinogen-secreting (chief) cells. Immunostaining of gastric-dispersed chief cells with a specific anti-PAR-2 antibody demonstrated expression of PAR-2 receptors on membrane...

Stepan, Vinzenz M., Dieter F. Krametter, Masashi Matsushima, Andrea Todisco, John Delvalle, and Chris J. Dickinson. Glycine-extended gastrin regulates HEK cell growth. Am. J. Physiol. 277 (Regulatory Integrative Comp. Physiol. 46): R572–R581, 1999.—Posttranslational processing of progastrin to a carboxy terminally amidated form (G-NH2) is essential for its effect on gastric acid secretion and o...

8-Amino-adenosine (8-NH2-Ado) is a ribose sugar nucleoside analogue that reduces cellular ATP levels and inhibits mRNA synthesis. Estrogen receptor-negative (ER ) metastatic breast cancers often contain mutant p53; therefore, we asked if 8-NH2-Ado could kill breast cancer cells without activating the p53-pathway. Regardless of the breast cancer subtype tested or the p53 status of the cells, 8-N...

Effective strategies to deal with rare earth pollution are urgently needed due the overexploitation of earths resource. In this study, a novel nanocomposite UiO-66-NH2/CPA-MA denoted as UA was successfully synthesized, which can simultaneously remove and detect Ce4+ in water. The hybrid consists UiO-66-NH2 CPA-MA. Based on high adsorption performance UiO-66-NH2, it capacity by adsorption. Moreo...

Previous studies have indicated that at least part of the selection of proteins for degradation takes place at a binding site on ubiquitin-protein ligase, to which the protein substrate is bound prior to ligation to ubiquitin. It was also shown that proteins with free NH2-terminal alpha-NH2 groups bind better to this site than proteins with blocked NH2 termini (Hershko, A., Heller, H., Eytan, E...

Abnormalities in repolarization of the cardiac ventricular action potential can lead to life-threatening arrhythmias associated with long QT syndrome. The repolarization process depends upon the gating properties of potassium channels encoded by the human ether-à-go-go-related gene (HERG), especially those governing the rate of recovery from inactivation and the rate of deactivation. Previous s...

Polyamidoamine dendrimers, which can deliver drugs and genetic materials to resistant cells, are attracting increased research attention, but their transportation behavior in resistant cells remains unclear. In this paper, we performed a systematic analysis of the cellular uptake, intracellular transportation, and efflux of PAMAM-NH2 dendrimers in multidrug-resistant breast cancer cells (MCF-7/...

The effect of plasma on degradation of human growth hormonereleasing hormone (GRH) was examined in vitro and in vivo using high performance liquid chromatography (HPLC), radioimmunoassay (RIA), and bioassay. When GRH(1-44)-NH2 was incubated with human plasma, the t1/2 of total GRH immunoreactivity was 63 min (RIA). However, HPLC revealed a more rapid disappearance (t1/2, 17 min) ofGRH(144)-NH2 ...