background: spinach is one of the vegetables with a high concentration of nitrate which is affected by various processes such as cooking and refrigeration before consumption. the present study aimed to investigate the effect of home cooking method, which has not been studied before, on the level of nitrate and nitrite in spinach. the effect of refrigeration was studied, as well.   methods: afte...

nitric oxide (no) is a molecule required for many physiological functions, produced from l-arginine by no synthases (nos). it is a free radical, producing many reactive intermediates that account for its bioactivity. sustained induction of the inducible form of nos (inos) in chronic inflammation may be mutagenic, through no-mediated dna damage or hindrance to dna repair, and thus potentially ca...

BACKGROUND Nitrite is a nitric oxide (NO) metabolite in tissues and blood, which can be converted to NO under hypoxia to facilitate tissue perfusion. Although nitrite is known to cause vasodilation following its reduction to NO, the effect of nitrite on platelet activity remains unclear. In this study, the effect of nitrite and nitrite+erythrocytes, with and without deoxygenation, on platelet a...

To understand the effects of physicochemical factors on nitrite transformation by microalgae, a lipid-rich Chlorella with high nitrite tolerance was cultured with 8 mmol/l sodium nitrite as sole nitrogen source under different conditions. The results showed that nitrite transformation was mainly dependent on the metabolic activities of algal cells rather than oxidation of nitrite by dissolved o...

Liquids treated with cold plasma emerged as ‘redox drugs’ in biomedicine, sources of reactive oxygen and nitrogen species targeting cellular functions, including wound healing cancer progression. The use cell culture media starting liquid, however, challenges the identification plasma-generated chemistry, limited by presence many organic compounds. Available detection methods need, therefore, t...

AIM To evaluate the levels of nitrite (NO(-)(2)) and nitrate (NO(-)(3)) ions and the incorporation of [(3)H]arachidonic acid (AA) into phospholipids of platelet membranes from coronary artery disease (CAD) patients with and without diabetes (NIDDM). SUBJECTS AND METHODS Eighteen CAD patients (group A), 18 CAD patients with NIDDM (group B), and 20 healthy controls (group C) without dyslipidemi...

An ion chromatographic method was developed for the determination of nine inorganic and organic acid anions at sub- to low-microg/l levels in power plant water samples. In this method, samples were injected using a large-volume direct injection technique, the analyte anions were separated on a hydroxide-selective anion-exchange column using high-purity hydroxide eluents generated by an on-line ...

Plasma nitrite and nitrate determinations are increasingly being used in clinical chemistry as markers for the activity of nitric oxide synthase and the production of nitric oxide radicals. However, a systematic evaluation of the determination of nitrite and nitrate in plasma has not been performed. In this study the recovery and stability of nitrite and nitrate in whole blood and in plasma, th...

This study assessed zooplankton density and Chl-a amount to understand their spatial seasonal variation in Lake Malombe. Samples were collected for analysis during the hot, dry season (HDS) cool, dry, windy (CDWS). The identified Tropodiaptomus cunningtoni (TC), Mesocyclops aequatorialis (MAA), Thermocyclops neglectus (TN), Bosmina longirostris (BL), Diaphanosoma spp. (DS), Nauplii. These group...

Nitrate uptake has been studied in nitrogen-deficient cells of the marine diatom Skektowema costatum. When these cells are incubated in the presence of nitrate, this ion is quickly taken up from the medium, and nitrite is excreted by the cells. Nitrite is excreted following classical saturation kineffcs, its rate being independent of nitrate concentration in the incubation medium for nitrate co...