Increasing pollution of the environment by nitrosocompounds has been observed. Acute intoxication of animals by nitrosocompounds leads to necrosis and chronic exposure to neoplasm. We have little information on the influence of nitrosocompounds on the metabolism of glycoconjugates (glycoproteins, glycolipids and proteoglycans) in animals. The aim of our work is to evaluate the influence of expo...

O6-Butylguanine was detected in the urine of rats given the butylating agent N-nitroso-N-butylurea. O6-Butylguanine contents in the 24-h rat urine samples after i.p. doses of 50, 100, and 200 mg/kg N-nitroso-N-butylurea were 1.03 +/- 0.41 (SE), 8.30 +/- 1.70, and 59.53 +/- 6.52 pmol, respectively. This suggests that O6-butylguanine formation in nucleic acids might be repaired in vivo, possibly ...

A native isolate of the colonial benthic diatom Staurosirella pinnata was cultivated for biosilica production. The silicified cell walls (frustules) were used as a source of homogeneous and structurally predictable porous biosilica for dye trapping and random laser applications. This was coupled with the extraction of lipids from biomass showing potential to fabricate photoactive composite mate...

The demethylation of carcinogenic N-nitrosodimethylamine (NDMA) and N-nitrosomethylaniline (NMA) is catalyzed by purified rat liver cytochromes P450 2B1 and 2B2 reconstituted with NADPH-P450 reductase and dilauroylphosphatidylcholine. A molar P450 to reductase ratio of about 1.0 is the most appropriate for the catalysis. NMA is a better substrate for both P450 enzymes than NDMA, with K(m) value...

An in vivo-in vitro alkaline DNA unwinding assay for single-strand breaks and/or alkali-labile lesions in hepatic DNA is described. The assay involves isolation of hepatic nuclei from mice, alkaline denaturation and unwinding of hepatic DNA, separation of single- and double-stranded DNA by hydroxylapatite batch chromatography, and quantitation of DNA in chromatographic fractions by a fluorometr...

Diabetes was induced in rats with Streptozotocin and alloxan and the tumorogenic effects of these two compounds were compared. One hundred thirty Lewis and SpragueDawley rats survived the induction of diabetes with Strepto zotocin. Fifty-six of these animals were sacrificed within 8 months of Streptozotocin administration and three were found to have renal tumors. Twenty-four of 74 (30.8%) Stre...

1) Microbial control is inadequate 2) It results in higher proportions of Mycobacterium, Pseudomonas, Acinetobacter and nitrifying bacteria in biofilms 3) It induces the viable-but-nonculturable (VBNC) state in Legionella 4) Higher concentrations of nitrite and nitrate (regulated drinking water contaminants) can occur 5) It degrades to ammonium ion in premise plumbing treatments which can lead ...

This study investigated the fate of eight N-nitrosamines during membrane bioreactor (MBR) treatment. The results suggest that biodegradation is mainly responsible for the removal of N-nitrosamines during MBR treatment. Other removal mechanisms were insignificant (e.g. adsorption to sludge) or not expected (e.g. photolysis and volatilization) given the experimental conditions and physicochemical...

The predominant nitrosamine observed in drinking water is N-nitrosodimethylamine (NDMA). NDMA may be formed via a nitrosation mechanism when sufficient nitrite is present and chlorine is primarily in the form of hypochlorous acid (HOCl). However, NDMA is typically not formed in the presence of free chlorine. NDMA may also be formed via a non-nitrosation mechanism in the presence of chloramines....

Abstract:N-nitrosaminesis a kind of poison which can induce cancer. Nitrite can react withsecondary amines to form N-nitrosamines in meat production. The objective of this experiment was to study N-nitrosamines formation ofthree kinds offermentationcelery powder (FCP506, FCP504, and FCP-homemade) and nitritein vitro nitrosation reaction system bydifferent pH, temperature, and the ratio of the s...