Nocardiosis is often misdiagnosed as tuberculosis in patients with HIV, as both diseases have similar manifestations. We describe the successful management of a case of advanced AIDS with disseminated Nocardial infection due to N. asteroides. Nocardial infection needs to be suspected in a patient with HIV infection when there is chest radiographic abnormality and when thrice sputum microscopy f...

Rabbits were sensitized with killed Mycobacterium tuberculosis, M. kansasii, M. avium, M. scrofulaceum, M. fortuitum, and Nocardia asteroides, and their response to homologous and heterologous antigens was assessed in vitro by direct and indirect macrophage migration inhibition tests. The antigens were obtained by disintegration of bacterial mass and by purification of the supernatants by ultra...

Isolation of Nocardia spp. from clinical specimens can be enhanced by the use of paraffin baiting, which relies on the selective ability of the organism to metabolize paraffin. We evaluated 44 Nocardia isolates, 18 group IV mycobacterial isolates, and 4 Streptomyces isolates for growth on blood agar (BA) and on carbon-free agar containing single or double substrates as follows: paraffin agar (P...

Fifty-two clinical isolates of Nocardia asteroides were tested by agar dilution for their susceptibility to 25 antimicrobial agents. In general, susceptibility could not be predicted based on the antibiotic class tested. However, the beta-lactams, including third-generation cephalosporins, were generally ineffective (MIC for 90% of the organisms [MIC90], between 64 and greater than 256 microgra...

Survival from murine pulmonary nocardiosis is highly dependent on CXC chemokine receptor-2 (CXCR2) ligand-mediated neutrophil chemotaxis and subsequent clearance of the infectious agent Nocardia asteroides. Intratracheal inoculation of N. asteroides rapidly up-regulated the CXC chemokines macrophage inflammatory protein-2 (MIP-2) and KC within 24 h, with levels remaining elevated through day 3 ...

The present study aimed to 1) determine the colonization rates of medically important Trichosporon species on normal perigenital skin and 2) determine the isolation rates of Trichosporon spp. isolated from the urine and catheters of Brazilian patients hospitalized in the Intensive Care Unit (ICU). The overall colonization rate of Trichosporon spp. was 11.15% (112 isolates). The most common spec...

Volatiles released by pathogenic and nonpathogenic mycobacteria, as well as by mycobacteria-related Nocardia spp., were analyzed. Bacteria were cultivated on solid and in liquid media, and headspace samples were collected at various times during the bacterial lifecycle to elucidate the conditions giving optimal volatile emission. Emitted volatiles were collected by using closed-loop stripping a...

A patient with severe non-healing microscopically proven Nocardia keratitis was treated with maximal topical amikacin followed by amniotic membrane transplantation (AMT) 2 months later (single-layer epithelial side-down). Epithelial healing was achieved, but neovascularization continued. This case report indicates that AMT combined with topical antibiotic provides pain relief and allows epithel...

It has been shown previously that aerobic actinomycetes can be separated into four main groups on the basis of their cell wall composition. Six representatives of aerobic actinomycetes (Nocardia asteroides and Micropolyspora brevicatena, cell wall type IV; N. madurae, Microbispora rosea, cell wall type III; Actinoplanes sp., cell wall type II; Streptomyces griseus, cell wall type I) were subjec...

A case of pulmonary nocardiosis is reported in a patient with malignant lymphoma and hypoimmunoglobuliwmia. The nocardiosis initially appeared as a dense infiltration in the right qper lobe, subsequently as bilateral long infiltrations. Nocardia asteroides was found in infralaryngeal secretion obtained by transtracheal aspiration. Treatment with sulfadine, lrimethoprim, and sulfamethoxazole (Se...