Mouse resident peritoneal macrophages display sufficient 5'-nucleotidase activity to hydrolyze 58 nm AMP/min per cell protein. This activity increases approximately 163 nm AMP/min per mg after 72 h in culture. The enzyme is renewed in unstimulated cells with a half-time of 13.9 h. The activity is not reduced by treatment of intact cells with a variety of proteolytic enzymes, including trypsin, ...

Objective: To evaluate the correlation between eosinophil differential count and total protein alkaline phosphatase levels in patients with normal high of aspartate aminotransferase from a polyclinic district Villa El Salvador Lima, Peru. Materials methods: An observational, analytical correlational study conducted apparently healthy male female aged 18 years or older, showing enzyme aminotrans...

Lysosomal arylsulfatases A and B of peripheral leukocytes from patients with chronic myelogenous leukemia and from healthy subjects were studied. Two enzyme activities of leukemia cells were significantly higher than those of cells from healthy subjects, irrespective of total and differential counts of leukemic cells. Upon anion-exchange chromatography, the arylsulfatases of chronic myelogenous...

background: glucose-6-phosphate dehydrogenase (g6pd) deficiency is the most common disease of the hexose monophosphate pathway existing in more than 400 million people worldwide. the aim of this study was to identify neonates with g6pd deficiency following national program for screening and education of affected newborns’ parents started since june 2007 in mazandaran, a northern province of ira...

abstract cyanide is a colorless, harmful, and dangerous substance with a smell similar to bitter almond. cyanide forms a stable compound with iron and magnesium and interferes with cell activity by interfering with respiration, carbon stabilization and nitrate revival. in order to evaluate cyanide metabolism in fuji and abassi mashhad apples, a factorial experiment was conducted as split plot b...

A 50% reduction in the activity of uroporphyrinogen-I (URO) synthase in liver, erythrocytes, and cultured skin fibroblasts characterizes all patients with clinically active acute intermittent porphyria (AIP). The same enzyme defect has also been demonstrated in the erythrocytes and skin fibroblasts of completely latent gene carriers of this disorder and presumably exists in the liver as well. I...

Three urea cycle enzymes, argininosuccinate synthetase, argininosuccinate lyase, and argÃ1⁄4Ã-ase, and two amino acyl-RNA synthetases, the glutamyl and the glutaminyl enzymes, have been studied in the Novikoff hepatoma and Morris Hepatomas 7787, 7793, and 7800. Except for Hepatoma 7793, which had "normal" activities of the 3 urea cycle enzymes, other hepatomas had lower activities than normal l...

The activities of hexokinase, citrate-cleavage enzyme, ;malic enzyme' and NADP-linked isocitrate dehydrogenase have been measured in the adipose tissue, testes and adrenals of normal rats, hypophysectomized rats and hypophysectomized rats treated with either thyroxine or thyroxine plus luteinizing hormone. Hypophysectomy reduced the activity of all four enzymes in all three tissues. Thyroxine a...

Three urea cycle enzymes, argininosuccinate synthetase, argininosuccinate lyase, and argÃ1⁄4Ã-ase, and two amino acyl-RNA synthetases, the glutamyl and the glutaminyl enzymes, have been studied in the Novikoff hepatoma and Morris Hepatomas 7787, 7793, and 7800. Except for Hepatoma 7793, which had "normal" activities of the 3 urea cycle enzymes, other hepatomas had lower activities than normal l...

The tritium release assay for the demonstration of thymidylate synthetase activity has been applied to the measurement of enzyme activity in the bone marrow of four patients with pernicious anemia and nine normal subjects. On the average, an approximately ninefold increase in enzyme activity was observed in patients with pernicious anemia. In the absence of 5, 10-methylene-tetrahydrofolate, enz...