The ability of a cyclodextrin-polyurethane polymer to remove ochratoxin A from aqueous solutions was examined by batch rebinding assays. The results from the aqueous binding studies were fit to two parameter models to gain insight into the interaction of ochratoxin A with the nanosponge material. The ochratoxin A sorption data fit well to the heterogeneous Freundlich isotherm model. The polymer...

Many cereal grains have been studied for their suitability as substrates for the fermentative production of mycotoxins. However, except for aflatoxin, wild rice has not been investigated. Hence, five mold cultures known to produce the mycotoxins ochratoxin-A, penicillic acid, patulin, vomitoxin, and zearalenone were grown on wild rice under varying conditions of moisture and temperature to dete...

The objective of the presented study was to develop and optimize a simple, high-throughput method for the control of 32 mycotoxins (Fusarium and Alternaria toxins, aflatoxins, ergot alkaloids, ochratoxins, and sterigmatocystin) in beer. Due to the broad range of their physicochemical properties, the sample preparation step was simplified as much as possible to avoid analyte losses. The addition...

The presence of toxin-producing fungi in foodstuffs and other agricultural commodities is well established (1-3). The Alternaria, Aspergilli, Fusaria and Penicillia have been repeatedly implicated as the pnncipal coinhabitants of products in which toxicity has been demonstrated (4,5). Numerous compounds have been isolated that can explain the toxicity of the Aspergilli, Fusaria, and Penicillia....

Forty five finished poultry feed samples, collected from different broilers, broiler breeders and layers farms were divided into two parts, for mycological and mycotoxins examination. In counting of molds, dilute plate technique was used, whereas feed parts were used for mycotoxin estimation, they were subjected to four standard kits of Aflatoxin, Ochratoxin, T-2 toxin and Fumonisins. Mold coun...

Samples of blood, kidney and liver per animal were randomly selected from slaughtered pigs (n=60) and analyzed for ochratoxin A. Determination of ochratoxin A (OTA) concentration in samples of kidney and liver was performed by HPTLC after immunoaffnity column clean-up, while for plasma samples, spectrofluorometric procedure was used. Of the 60 plasma samples, 60% contained OTA in the range of 2...

Aptamers have been used as recognition elements for several molecules due to their great affinity and selectivity. Additionally, mesoporous nanomaterials have demonstrated great potential in sensing applications. Based on these concepts, we report herein the use of two aptamer-capped mesoporous silica materials for the selective detection of ochratoxin A (OTA). A specific aptamer for OTA was us...

The OTA content of 30 roasted coffees purchased in French supermarkets was evaluated by two validated different methods: one using immunoaffinity column (IAC) clean-up after alkaline extraction; the second using toluene extraction under acidic conditions. OTA recoveries (0.5 to 5 µg/kg) ranged from 16-49% with the alkaline extraction method and 55-60% with the acidic method. OTA recoveries from...

Healthy blood donors from the city of Zagreb were checked for the presence of a nephrotoxic mycotoxin ochratoxin A (OTA) in the plasma. Samples of blood were collected in June, September, and December 1997, and March 1998, totalling 200 or 50 in each round. The concentrations of OTA were measured using high pressure liquid chromatography (HPLC) method (detection limit 0.2 ng OTA/ml of plasma). ...

The in vitro and in vivo evidence compatible with a role for oxidative stress in OTA carcinogenicity has been collected and described. Several potential oxido-reduction mechanisms have been identified in the past. More recently, the possibility of a reduction of cellular antioxidant defense has been raised as an indirect source of oxidative stress. Consequences resulting from the production of ...