A process for pea-protein isolate production, resulting in low content of phytate and oligosaccharides, has been developed. Oligosaccharides were removed from the protein fraction through ultrafiltration. Ultrafiltration of 50- and 100-kD molecular-weight cutoffs (MWCOs) were tested, and both effectively separated the oligosaccharides from the protein. Phytate degradation was achieved by incuba...

AIMS To determine whether alternansucrase (ASR)-derived oligosaccharides can support the in vitro growth of various intestinal bacteria. METHODS AND RESULTS Growth was assessed from each culture after incubation in a medium containing ASR-derived oligosaccharide as sole carbohydrate source. Most of the Bifidobacterium spp. tested showed growth on all five of the oligosaccharides tested while ...

The carbohydrate binding specificity of Allomyrina dichotoma lectin II was investigated by analyzing the behavior of various complex type oligosaccharides and human milk oligosaccharides on an A. dichotoma lectin II-agarose column. Basically, the lectin interacts with the Gal beta 1----4GlcNAc group. Substitution of their terminal galactose residues by Neu5Ac alpha 2----6 will enhance their aff...

Intestinal colonization is beneficial to the role of probiotics, and prebiotics can promote adhesion probiotics in intestine. This study optimized combination complex that could improve growth ability Limosilactobacillus fermentum (L. fermentum) DALI02 Caco-2 cells vitro determined effect its quantity time immunocompromised rat model. The results showed all five (fructo-oligosaccharides (FOS), ...

Breastfeeding is essential in the first months of a newborn’s life. Breast milk source crucial macronutrients, prebiotic oligosaccharides, and potential probiotic strains bacteria. Oligosaccharides from breast (HMOs) are significant part composition represent complex digestible sugars. This study aims to elucidate enzymatic hydrolysis these oligosaccharides other prebiotics by bacteria present ...

The structure of carbohydrate units of rat colonic mucus glycoproteins was investigated. Alkaline borohydride reductive cleavage of the glycoprotein resulted in the release of a series of reduced oligosaccharides. Eight of the oligosaccharides (1 to 8), ranging in length from 2 to 12 sugar units, have been purified to homogeneity and their structures determined. Based on the results of immunolo...

The structures of a series of large oligosaccharides derived from acharan sulfate were characterized. Acharan sulfate is an unusual glycosaminoglycan isolated from the giant African snail, Achatina fulica. Oligosaccharides from decasaccharide to hexadecasaccharide were enzymatically prepared using heparin lyase II and purified. Capillary electrophoresis and gel electrophoresis confirmed the pur...

Prebiotic oligosaccharides, with a degree of polymerization (DP) of mostly less than 10, exhibit diverse biological activities that contribute to human health. Currently available prebiotics are mostly derived from disaccharides and simple polysaccharides found in plants. Subtle differences in the structures of oligosaccharides can cause significant differences in their prebiotic proper-ties. T...

Establishing the analytical platforms for characterizing human milk oligosaccharides is important to fully assess their specific functionalities. The characterization of these biomolecules, however, is still considered challenging, owing to their overall complexity and diversity. Addressed here are the technical difficulties with an emphasis on the application of mass spectrometry to rapidly pr...

DE protection, demineralization, de colorization, and acetylation. Protein accesses were used to obtain chitosan from shrimp shell waste. Using FTIR, SEM XRD. It was characterized. Also analyzed the physiochemical parameter such as moisture content, hydrogen meter, viscosity, degree of acetylation solubility The age bacterial culture influenced its susceptibility with cells being most prone in ...