A method is described for the separation of the outer membrane (OM) from the cytoplasmic membrane (CM) of Pseudomonas cepacia grown in nutrient broth and in chemically defined media under different nutrient depletions. The method is particularly valuable since it is effective when applied to stationary phase cells. Enzyme activities indicated that the contamination of the OM with the CM was les...

T3 regulates energy metabolism, glucose and lipid metabolism and several markers of adipocyte differentiation in white adipose tissue (WAT). T3 levels in tissues are regulated by the deiodinases (D1, D2, D3 or DIO1, DIO2, DIO3, respectively). Aim: To analyze DIO1, DIO2 and DIO3 deiodinases activity and mRNA expression and T4 and T3 concentrations in omental (OM) and subcutaneous (SC) WAT from o...

Otitis media (OM), a middle-ear infection, is the most common childhood illness treated by pediatricians. If inadequately treated, OM can result in long-term chronic problems persisting into adulthood. Children with chronic OM or recurrent OM often have conductive hearing loss and communication difficulties and require surgical treatment. Tympanostomy tube insertion, the placement of a small dr...

Decreased expression of cardiac myosin binding protein-C (cMyBP-C) in the myocardium is thought to be a contributing factor to hypertrophic cardiomyopathy in humans, and the initial molecular defect is likely abnormal cross-bridge (XB) function which leads to impaired force generation, decreased contractile performance, and hypertrophy in vivo. The myosin activator omecamtiv mecarbil (OM) is a ...

Four ruminally and duodenally cannulated Suffolk wether lambs (34.5 +/- 2.0 kg initial BW) were used in a 4 x 4 Latin square-designed experiment to examine the effects of ruminal protein degradability and supplementation frequency on site and extent of digestion in lambs consuming a low-quality forage diet. Wethers were fed a basal diet of mature crested wheatgrass hay (4.2% CP) for ad libitum ...

In the Rio Negro basin, podzols develop at the expense of clay-depleted laterites through localised and spectacular weathering fronts. This natural process leads to the remobilization of previously accumulated organic matter (OM) and elements (Al, Fe, Si). OM is thus redistributed within soil profiles and exported towards rivers, giving them their typical black coloration. In soils, this redist...

The changing climate in the Arctic has a profound impact on permafrost coasts, which are subject to intensified thermokarst formation and erosion. Consequently, terrestrial organic matter (OM) is mobilized and transported into the nearshore zone. Yet, little is known about the fate of mobilized OM before and after entering the ocean. In this study we investigated a retrogressive thaw slump (RTS...

The need for accurate information on the organic matter (OM) content in soils at European, National or Regional level has been increasing steadily over the past few years. This is a result of increasing concern about environmental problems such as soil degradation, desertification (CEC, 1992; UNEP, 1991), erosion and, at the worldwide level, the impact of climate change. There is some evidence ...

introduction: the aim of the present in vitro study was to evaluate the efficacy of d-race, protaper retreatment kit and hand h-files in removal of obturating materials (om) from the curved root canals using cone-beam computed tomography (cbct). materials and methods: sixty extracted molars were prepared and obturated. the samples were divided into three groups ( n =20). in each group the om wa...