A comparison was made of the esterase and activator activities of the various activated forms of human plasminogen and their streptokinase complexes with Nalpha-Cbz-L-lysine-p-nitrophenyl ester as the substrate. The steady state kinetic properties of Glu- and Lys-plasmins, and Glu- and Lys-plasminogen-streptokinase complexes were identical, while the Lys-plasmin-streptokinase complex showed a 2...

Purified bovine rhodopsin was reconstituted into vesicles consisting of 1-stearoyl-2-oleoyl phosphatidylcholine or 1-stearoyl-2-docosahexaenoyl phosphatidylcholine with and without 30 mol % cholesterol. Rhodopsin stability was examined using differential scanning calorimetry (DSC). The thermal unfolding transition temperature (T(m)) of rhodopsin was scan rate-dependent, demonstrating the presen...

Employing the graded versions of the Yang-Baxter equation and the reflection equations, we construct two kinds of integrable impurities for a small-polaron model with general open boundary conditions: (a) we shift the spectral parameter of the local Lax operator at arbitrary sites in the bulk, and (b) we embed the impurity fermion vertex at each boundary of the chain. The Hamiltonians with diff...

A kinetic priority queue is a kinetic data structure which determines the largest element in a collection of continuously changing numbers subject to insertions and deletions. Due to its importance, many different constructions have been suggested in the literature, each with its pros and cons. We propose a simple construction that takes advantage of randomization to achieve optimal locality an...

One of the open problems posed in [3] is: what is the minimal number k such that an open, flexible k-chain can interlock with a flexible 2-chain? In this paper, we establish the assumption behind this problem, that there is indeed some k that achieves interlocking. We prove that a flexible 2-chain can interlock with a flexible, open 16-chain.

Using singly gapped or nicked templates containing the T7A1 promoter, we have measured several kinetic parameters related to the process of transcription initiation by Escherichia coli RNA polymerase, confirming and extending previous results using a population of randomly gapped templates. A reduced probability of transcript abortion at RNA lengths of 6 and 7 nucleotides and a lower ratio of a...

Substrate determinants for rabbit and chicken skeletal muscle myosin light chain kinases were examined with synthetic peptides. Both skeletal muscle myosin light chain kinases had similar phosphorylation kinetics with synthetic peptide substrates. Average kinetic constants for skeletal muscle myosin light chain heptadecapeptide, (formula; see text) where S(P) is phosphoserine, were Km, 2.3 micr...

The branched chain ammo acid aminotransferases (transaminase B) of Salmonella typhimurium and Salmonella montevideo have been purified and studied kinetically. The results of partial kinetic characterization of the enzyme support the conclusion that the reaction proceeds via a binary “ping-pang” mechanism. The Michaelis constants for the amino acceptor, cr-ketoglutarate, and three branched chai...

Anatomical studies describe the vastus medialis (VM) as being subdivided into two morphologically distinct components, obliquus (VMO) and longus (VML). However, there are discrepancies regarding functional differentiation of these components. The aim this study was to compare levels activation VMO VML by high density surface electromyography. Twelve healthy young women (age: 21.4 ± 2.0 years; ...

results we identified no mutations in these patients in the three screened positions. conclusions to ensure that these genes play no role in the disease, evaluation of the non-coding regions of both the cyp1b1 and myoc genes is strongly recommended, since other genes are involved in the pathogenesis of glaucoma. patients and methods forty patients with poag were recruited from the ophthalmic di...