In vitro activation of dark-inactivated pyruvate, orthophosphate dikinase extracted from maize (Zea mays L. cv. Golden Cross Bantam T51) leaves was examined. The inactive form of the enzyme and orthophosphate behaved kinetically as substrates for the reaction, which was catalyzed by an activating factor. This factor was bound by Blue Dextran Sepharose 4B and could be eluted by KCl at a concentr...

Disodium hydrogen orthophosphate is a water soluble white powder widely used as pH regulator and saline laxative. The sodium nitrate is a highly water soluble white solid, used in high blood pressure, dentinal hypersensitivity, and production of fertilizers. The present study was aimed to investigate the impact of biofield treatment on spectral properties of disodium hydrogen orthophosphate and...

Historically, it was assumed that reactive, inorganic phosphorus present in pristine environments was solely in the form of orthophosphate. However, this assumption contradicts theories of biogenesis and the observed metabolic behavior of select microorganisms. This paper discusses the role of ion chromatography (IC) in elucidating the oxidation-reduction cycle of environmental phosphorus. Thes...

Adenylate deaminase from rat skeletal muscle has been studied with the objective of understanding how the activity of the enzyme is regulated in uiuo. ATP and GTP inhibit the enzyme at low concentrations in the presence of 150 mM KCl. The ATP inhibition is reversed as the ATP concentration is raised to physiological levels. The G’l’P inhibition is reversed as the GTP concentration is raised to ...

This study investigated the chemical and thermal properties of faecal sludge from 10 dry VIP latrines in Bester’s Camp in the eThekwini Municipality, Durban, South Africa. Faecal sludge samples were selected at different depths and from the front and back sections of 10 VIP latrines during a manual emptying process. The samples were analysed for: moisture content; volatile solids; chemical oxyg...

We have investigated the increased rate of glycolysis following transformation of chick embryo fibroblasts by Rous sarcoma virus. In both normal and transformed cells the regeneration of adenosine 5 -diphosphate and orthophosphate was found to be the rate-limiting factor for glycolysis. The plasma membrane Na -K -adenosine5 -triphosphatase (ATPase) was a major contributor to adenosine 5 -diphos...

After inoculation of a stationary-phase culture of Nitrosomonas europaea into fresh growth solution, the cell-associated orthophosphate increased rapidly to 800 mumoles/g (wet weight), whereas the acid-insoluble long-chain polyphosphate content decreased rapidly to 22 mumoles/g. As growth proceeded, the orthophosphate content decreased rapidly to a level of 15 mumoles/g and the long-chain polyp...

The present study was carried out to evaluate the phytoplankton community and water quality in four parallel fishponds (FP1 to FP4) during one year. Some abiotic parameters of water column and phytoplankton was sampled in four sites, one in each fishpond. Similarity analysis showed that FP2 fishpond was markedly different for the others ponds with regard to phytoplankton community. Conductivity...