BACKGROUND Loss of meniscal tissue is correlated with early osteoarthritis but few data exist regarding detailed biomechanical properties (e.g. viscoelastic behavior) of menisci in different species commonly used as animal models. The purpose of the current study was to biomechanically characterize bovine, ovine, and porcine menisci (each n = 6, midpart of the medial meniscus) and compare their...

Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...

Fatty acid oxidation is an important energy source for the oocyte; however, little is known about how this metabolic pathway is regulated in cumulus-oocyte complexes. Analysis of genes involved in fatty acid oxidation showed that many are regulated by the luteinizing hormone surge during in vivo maturation, including acyl-CoA synthetases, carnitine transporters, acyl-CoA dehydrogenases and acet...

The mammalian oocyte develops within a complex of somatic cells known as a follicle, within which signals from the somatic cells regulate the oocyte, and signals from the oocyte regulate the somatic cells. Because isolation of the oocyte from the follicle disrupts these communication pathways, oocyte physiology is best studied within an intact follicle. Here we describe methods for quantitative...

The oocyte is a highly differentiated cell. It makes organelles specialized to its unique functions and progresses through a series of developmental stages to acquire a fertilization competent phenotype. This review will integrate the biology of the oocyte with what is known about oocyte-specific gene regulation and transcription factors involved in oocyte development. We propose that oogenesis...

概要 In vivoで 肺血管外水分量(pulmonary extravascular water volume, PEV)を測 定す るこ とは,1954年Chinard & Ennsに よ り示唆 されて以来,基 礎的検討がか さね られ臨床への応用が可 能 となつた.本 論文 は,Ramseyら の方法 に準 じて後天性 心疾患に おけ るPEVを 測定 し,肺 循環諸 量,臨 床経過 お よび血液 ガス値 との関連について検 討 した ものである。測定時 の肺血管内圧 とPEV とは,粗 な正相 関(r=0.48,P<0.02)を 示 すにす ぎず,む しろPEVは 左心不全の既往 と関連 が あ る とい う結果 をえた.す なわ ち測定時 の肺 血管 内圧は同 じレベ ルにあ りなが ら,左 心不 全既往のあ る例は,既 往 のない例 に比ベ てPEVは 著 明な増加を示...

BACKGROUND The complexity of fertilization failure during assisted reproductive technologies (ART) is often under-appreciated, as this failure can occur at any number of essential mechanistic and cellular events. Importantly, successful fertilization is heavily dependent upon inherent qualities of the oocytes, and thus reliant upon fidelity of oocyte maturation. METHODS Pubmed and medline wer...

Accession Number. U15731. Species. Bos taurus. Origin of Clone. Hybridizing with a 220-bp cDNA of the somatotropin receptor (Hauser et al., 1990) 10 clones were identified from a bovine genomic library (EMBL3 SP6pT7 cloning vector; Clontech Laboratories, Palo Alto, CA). A 2.9-kb XhoIIAatII fragment containing the promoter and exon I was subcloned into pGEM-7Zf (Promega, Madison, WI), and both s...

Recent findings from this laboratory suggest that the biological actions of placental lactogen (PL) in mammalian fetal tissues are mediated through binding of the hormone to a distinct and unique PL receptor. We have now purified this receptor from fetal and maternal sheep liver, characterized its binding to PL, growth hormone (GH), and prolactin (PRL), and determined its molecular weight by SD...

Staphylococcus aureus is an important pathogen of many species, including sheep, and impacts on both human and animal health, animal welfare, and farm productivity. Here we present the widest global diversity study of ovine-associated S. aureus to date. We analysed 97 S. aureus isolates from sheep and sheep products from the UK, Turkey, France, Norway, Australia, Canada and the USA using multil...