1. The effect of pyridoxal 5'-phosphate on the activity of ox liver glutamate dehydrogenase towards different amino acid substrates was investigated. 2. Both alanine and glutamate activities decreased steadily in the presence of pyridoxal 5'phosphate. 3. The alanine/glutamate activity ratio increased as a function of inactivation by pyridoxal 5'-phosphate, indicating that glutamate activity is ...

The electrochemistry of tellurium in two different deep eutectic solvents, choline chloride - urea (ChCl-U) and oxalic acid (ChCl-Ox) was studied on glassy carbon (GC) polycrystalline (Au(poly)) gold electrodes by cyclic hydrodynamic voltammetries. Voltammetric responses associated with the redox pairs Te(IV)/Te(0) Te(0)/Te(-II) are recorded, a striking difference between ChCl-U ChCl-Ox being o...

A single-center randomized, controlled cross-over exercise intervention in 20 women willing to reduce weight (20–40 y, BMI: 27.4 ± 2.1), with the aim examine potential benefits for loss under normal (N-Ox: 20.9%) and mildly reduced (R-Ox: 17.0%) normobaric oxygen an “Altitude Simulation Chamber”. O2 consumption (VO2), CO2 production (VCO2), blood saturation (SaO2), glucose lactate (mM) were stu...

Dietary oxalate plays a very important role in the formation of calcium oxalate stones, and dietary intake of calcium may decrease oxalate absorption and its subsequent urinary excretion. The purpose of the present study was to determine the effect on urinary oxalate excretion of an acute oral calcium load, standard milk, or high-calcium low-fat milk followed by a dose of oxalic acid. Male Wist...

BACKGROUND Exposure of renal epithelial cells to oxalate (Ox) or calcium oxalate (CaOx) crystals leads to the production of reactive oxygen species and cell injury. We have hypothesized that Ox and CaOx crystals activate NADPH oxidase through upregulation of its various subunits. METHODS Human renal epithelial-derived cell line, HK-2, was exposed to 100 μmol Ox or 66.7 μg/cm(2) CaOx monohydra...

CD36 is a multifunctional cell-surface receptor that binds adhesion molecules such as thrombospondin-1 and collagen and modified lipids and/or lipoproteins. It participates in cellular uptake of photoreceptor outer segments and scavenging of apoptotic cells and oxidized low density lipoprotein (Ox-LDL). Recognition and internalization of Ox-LDL by mononuclear phagocytes may play an important ro...

The present work investigated the mechanism for down-regulation of thrombomodulin (TM), an anticoagulant glycoprotein, on cultured umbilical vein endothelial cells (HUVECs) exposed to lipid extracts from oxidized low-density lipoprotein (oxLDL). HUVECs exposed to phospholipid extracts, but not to free cholesterol, triglyceride, or cholesterol ester, isolated from ox-LDL reduced TM mRNA levels t...

We investigated whether the normal anabolic effects of acute hyperaminoacidemia were maintained after 5 days of oxandrolone (Oxandrin, Ox)-induced anabolism. Five healthy men [22 +/- 3 (SD) yr] were studied before and after 5 days of oral Ox (15 mg/day). In each study, a 5-h basal period was followed by a 3-h primed-continuous infusion of a commercial amino acid mixture (10% Travasol). Stable i...

We investigated the impact of growth hormone (GH) alone, testosterone (T) alone, and combined GH and T on whole body protein metabolism. Twelve hypopituitary men participated in two studies. Study 1 compared the effects of GH alone with GH plus T, and study 2 compared the effects of T alone with GH plus T. IGF-I, resting energy expenditure (REE), and fat oxidation (F(ox)) and rates of whole bod...