Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. All of the P.multocida isolates were recovered from fowl cholera outbreaks in Northern provinces of Iran. Amplification was based on the gene ompH, encoding a major outer membrane protein (protein H). PCR with ompH primers amplified...

Mice immunized with the potassium thiocyanate extract of Pasteurella haemolytica, serotype 1, were found to resist a challenge infection of P. multocida type A, thus demonstrating cross-protection. This finding was further supported by the finding that an antiserum directed against the potassium thiocyanate extract of P. haemolytica was bactericidal to P. multocida and vice versa.

A supplemented 2% peptone broth is described for the detection of indole production by Pasteurella multocida. The 96 isolates of P. multocida that were utilized in this evaluation were indole positive within 18 to 24 h.

A specific, rapid plate agglutination procedure for serotyping Pasteurella haemolytica was performed. The procedure did not require special antigen preparation and yielded essentially the same results as the indirect hemagglutination procedure.

Peripheral blood and splenic lymphocytes from rabbits immunized with Pasteurella multocida by various schedules were stimulated to undergo blast transformation in vitro in response to this antigen. Repeated immunizations suppressed this response.

Chemically defined media for the growth of Pasteurella tularensis have been described by Tamura and Fleming (1949) and more recently by Giesken (1954), Mager et al. (1954), and Traub et al. (1955). Upon application of the latter medium (Traub et al.) for the growth of a virulent strain of P. tularensis, it was noted that the viable cell yields were markedly lower than yields previously obtained...