Faecal samples were collected from 573 slaughtered cattle aged between three and 24 months in seven abattoirs. After enrichment (mTSB with novobiocin), samples were screened by real-time PCR first for stx and if positive, tested for the top-five Shiga toxin-producing Escherichia coli (STEC) serogroups using PCR assays targeting genes specific for serogroups O26, O103, O111, O145 and O157. Of 56...

An “In house” PCR method was compared with three commercial PCR kits for the detection of Mycoplasma gallisepticum (MG) in cultures grown in a modified Frey broth or agar, in vaccines, as well as in tracheal swabs of SPF chickens infected experimentally. The studied methods showed different specificity (poor specificity of commercial A test) and sensitivity. The “in house” method appeared to be...

Zoonotic visceral leishmaniasis (VL) is endemic in northwestern Iran. Real-time PCR, conventional PCR, and the direct agglutination test (DAT) were used to diagnose Leishmania infantum infection in blood samples from 100 domestic dogs and 100 humans. Based on clinical evaluation, 82 humans and 72 dogs from the endemic area were categorized as having asymptomatic infection, DAT positive with no ...

background: cryptosporidium parvum is a zoonotic pathogen transmissible from a variety of animals to humans and is a considerable public health concern. dairy cattle have been identified in numerous reports as a major source of environmen-tal contamination with this pathogen. the aim of study was to detect and isolate the cryptosporidium spp . from fecal samples of naturally infected pre-wean c...

The current study aims to detect the presence of some virulence genes Escherichia (E.) coli in fish. 46 strains E. were previously isolated from 153 fish samples, including 28 and 18 isolates local markets farms, respectively, Nineveh Governorate November 2021 January 2022. results show that all possessed uidA gene with a molecular weight 623 bp. In addition, they 88.9% (16/18) farmed samples S...

Background: Avian chlamydiosis is a zoonotic disease ofbirds caused by the intracellular bacterium Chlamydia psittaci. Avianchlamydiosis leads to severe respiratory disease in young turkeys and eggproduction losses in layers. OBJECTIVES: Due to paucity of information about theprevalence of chlamydial infection in the turkey population in Iran, this studywas conducted to detect chlamydial infect...

Otosclerosis is a localized bone dystrophy of unknown etiology mainly involving the stapes. The hypothesis of a persistent infection by the measles virus was based on the inconstant detection of the virus by various methods, including reverse transcription-PCR (RT-PCR) of patients' stapes samples. The aim of this work was to investigate the presence of the measles virus in stapedial otosclerosi...

abstract: ornithobacterium rhinotracheale (ort) has been identified as one of the emerging respiratory bacterial pathogens in turkey and chicken flocks. diagnosis of ort infection has been faced some difficulties in isolation and identification of the bacterium based on the biochemical properties. a reliable identification method that can be used in routine laboratory investigations is of impor...

Aflatoxins are secondary toxic metabolites produced by some Aspergillus spp. particularly, Aspergillus flavus and A. parasiticus that contaminate food and feed. The objective of this study was to evaluate the contamination of feedstuffs with Aspergillus spp. and detect genes involved in the aflatoxin biosynthesis pathway of A. flavus and A. parasiticus isolates. A total of 110 cow feed samples ...