the polymerase chain reaction (pcr) as applied to detection of a foreign dna in clinical specimens could provide a sensitive instrument for rapid detection of viral dna persisting in tissues of patients suspected of latent infection. human cytomegalovirus (hcmv) dna was found in arterial plaques of patients with atherosclerotic lesions using a pcr assay with nested primer oligonucleotides deriv...

background and objectives: urinary tract infection (uti) is one of the most common infections and a major cause of patient morbidity world wide. the gold standard for detection of bacterial pathogens in uti is culture of urine specimens. in order to facilitate identification of bacterial pathogens in clinical urine specimens we designed a molecular method to detect bacterial pathogen in uti. ma...

PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of vario...

Outer membrane proteins (OMPs) are the major virulent factors of Haemophilus parasuis. PCR-RFLP targeting the ompA gene was conducted to investigate the possibility of genotyping H. parasuis in this study. Fifteen reference strains and 49 isolates from pig farms in northwest China were genotyped by PCR-RFLP with a pair of specific primers. The results indicated that both the 15 reference strain...

T-RFLP clone characterization (screening) was optimized for a fast and basepair-accurate characterization of clones from marine Archaea collected from the Eastern Mediterranean Sea. Because of the high sensitivity of T-RFLP fingerprinting, a protocol was developed where 10 initial PCR cycles gave detectable terminal fragments from clones. Additionally, forward and reverse primers for PCR were i...

Recent studies have shown the usefulness of PCR-based restriction fragment length polymorphism (RFLP) analysis for differentiating Helicobacter pylori strains isolated by culture. For this study, a PCR-based RFLP assay was developed for directly typing H. pylori strains from gastric biopsy specimens. Nineteen gastric biopsy specimens obtained from patients undergoing endoscopy for gastrointesti...