We report two of our recent efforts for improvement of PCR detection and genomic analysis of Xylella fastidiosa (Xf). We evaluated the use of PCR primers from a hyper-variable locus to monitor diversity within strains of the same pathotype. The pspB (PD1208) locus, encoding a serine protease, was selected and analyzed. It was observed that tandem repeat sequences in pspB locus were highly varia...

This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by ...

Tubulysins are a group of secondary metabolites produced by myxobacteria that inhibit the function eukayotic cytoskeleton. We developed pair PCR primers specifically amplified tubulysin biosynthetic genes. Using these primers, eight out eighty-one strains belonging to Cystobacteraceae family harbored putative genes were screened through analysis. The selected included two Archangium gephyra, St...

background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr.  materials and methods: genomic dna from bacillus thermoca...

This paper presents a hierarchical DNA memory based on nested PCR. Each memory consists of address blocks and a data block. In order to access specific data, we specify the order of the address primers, and nested PCR are performed by using these primers. Our laboratory experiments are also presented to demonstrate the feasibility of the proposed memory.

In the present study sensitivity of diagnosis of rabies with Nested RT-PCR was compared with Immunofluorescence on 20 brain samples. RNA extraction was done using Qiazol method. Synthesis of cDNA was done using rabies specific primers. Nested set of primers were used to amplify highly conserved 762bp nucleoprotein gene region. Nested RT-PCR was able to diagnose rabies viral RNA in 12 out of 13 ...