rapid and accurate identification of microorganisms have a significant impact on strategies and fish health management programs. hence, in this study a duplex pcr assay based on the 16s rrna gene for simultaneous detection of aeromonas hydrophila rticc 1032 and escherichia coli rticc 2325 from pure cultures, and challenged fish tissues was performed and their results were compared with the resu...

A high percentage of pleural effusions remain unexplained despite an intensive diagnostic workup. Epstein-Barr virus (EBV) infections occur worldwide and affect the majority of the population. The present study investigated the prevalence and clinical relevance of EBV in pleural effusions. A prospective study was performed in which 60 consecutive patients with pleural effusion were enrolled. Re...

Laser capture microdissection (LCM) allows microscopic procurement of specific cell types from tissue sections that can then be used for gene expression analysis. In conventional LCM, frozen tissues stained with hematoxylin are normally used to the molecular analysis. Recent studies suggested that it is possible to carry out gene expression analysis of formaldehyde-fixated paraffin embedded (FF...

BACKGROUND Microbiological investigations of vitreous fluid (VF) and aqueous humour (AH) specimens have often failed to detect the infecting agent in infectious endophthalmitis, resulting in a clinical dilemma regarding therapy. In this study, the polymerase chain reaction (PCR) was evaluated in the diagnosis of bacterial and Propionibacterium acnes endophthalmitis. METHODS 58 intraocular spe...

report on a series of 52 excised heart valves on which they performed broad-range polymerase chain reaction (PCR) to identify microbes in valve tissues that were defined as having infective endocarditis (IE). Internal sequencing of amplicons, with specific “nested” primers to identify microbial subspecies, was performed after broad-range PCR. The authors report that on the basis of gross featur...

Myclobutanil is a chemical pesticide commonly used in the production of some vegetables and fruits like greenhouse peppers, grapes, apples. The aim this study was to investigate genotoxic cytotoxic effects myclobutanil fungicide on Allium cepa plant, model organism. Randomly amplified polymorphic DNA inter simple sequence repeat-PCR techniques were performed A. exposed different doses time peri...

Background: This study aimed to design and analyze the applicability of an oligonucleotide probe in radioisotope 32P-based dot blot hybridization for detection of hepatitis C virus. Methods: Forty-six of plasma samples were used. The plasma was extracted to obtain viral RNA genome as template for RT-PCR and the amplicon was used for nested PCR. Twenty-four HCV genomes were retrieved from GeneBa...

SARS-COV-2 is a deadly virus which emerged in China late 2019. In this article, the difference European variant (2nd wave) and UK (3rd of COVID-19 Pakistan was investigated. Polymerase chain reaction (PCR) technique applied to evaluate gene sequence both variants. The more terrible due its resistance genomic mutation. When performed PCR waves, there nucleocapsid protein (N gene) while envelop (...

INTRODUCTION Diagnosis of brucellosis in Syria is based on the presence of compatible symptoms in addition to positive agglutination results. This study investigated the potential of culture, ELISA and real-time PCR to support the diagnosis in different clinical manifestations of brucellosis. METHODOLOGY Peripheral blood samples from 34 suspected brucellosis patients and 42 probable chronic o...