Methods Peanut allergy was induced in mice on the C57Bl/6 background (IA) with 4 weekly oral gavage feedings of peanut protein and cholera toxin. After a two-week rest period, sensitized animals were challenged by intraperitoneal injection with crude peanut extract (CPE) and monitored for anaphylaxis. Clinical indicators of peanut allergy include decreased body temperature, scratching, swollen ...

BACKGROUND IgE-mediated peanut allergy is a complex trait with strong heritability, but its genetic basis is currently unknown. Loss-of-function mutations within the filaggrin gene are associated with atopic dermatitis and other atopic diseases; therefore, filaggrin is a candidate gene in the etiology of peanut allergy. OBJECTIVE To investigate the association between filaggrin loss-of-functi...

BACKGROUND Diagnosing peanut allergy based on sensitisation alone leads to an unacceptable rate of overdiagnosis. OBJECTIVE To define parameters that may help differentiate peanut allergy from asymptomatic sensitisation in a cohort of South African (SA) children with atopic dermatitis (AD). It is the first study in SA to utilise oral food challenge tests and analyse peanut component patterns....

Background: Aflatoxins (AFs) are natural toxins produced by fungus belonging to genus Aspergillus. These toxins are the secondary metabolites, which may cause teratogenic, mutagenic, and carcinogenic effects due to contamination of food. Peanut is an economically important crop, grown in many parts of the world. The main aim of this survey was to detect AFs in peanut oils marketed in Peshawar, ...

Methods We included 60 subjects in different groups: Group 1, peanut and peach allergy; Group 2, peanut allergy and good tolerance to peach; Group 3, peach allergy and good tolerance to peanut; Group 4, good tolerance to peach and peanut. The diagnostic was performed by clinical history, skin prick test and/or double blind placebo controlled food challenge. BAT to Arah1, Arah2, Arah3, Arah6, rA...

Peanuts, cottonseed, rice, and sorghum from Texas were sampled over a 3-year period. To insure adequate isolation of alfatoxin-producing species of fungi, low-quality lots were sampled at a rate greater than their respective proportional representation. Aflatoxins were found each year in peanut and cottonseed and were found in 2 of 3 years in rice and sorghum. Aflatoxins were detected in all fo...

Inadvertent exposure to peanut in foods poses health risks for peanut-allergic individuals that can be reduced by improving detection systems for allergen contaminants in food products and manufacturing processes. Detection of peanut in chocolate has been especially difficult. We report the optimization of conditions for measuring a major peanut allergen, Ara h 1, in chocolate with the use of a...

Methods DBPCFC was performed with increasing concentrations of peanut allergen (1 mg to 5 g of peanut) in 42 children with suspected IgE-mediated peanut-allergy. Blood samples were taken for analyses of CD-sens and quantification of IgE-antibodies to peanut and Ara h 1-3, 8-9. Basophils were stimulated in vitro with peanut allergen in descending doses until the threshold sensitivity was reached...

BACKGROUND The relative contribution and the relation between individual peanut allergens in peanut allergic responses is still matter of debate. We determined the individual contribution of peanut proteins to B, T cell and allergic effector responses in a mouse model for peanut allergy. METHODS Mice were immunized and challenged by oral gavage with peanut protein extract or isolated allergen...

BACKGROUND Peanut allergy is an increasingly common health problem. Current treatment guidelines are based on strict avoidance. However, in the last few years, oral immunotherapy protocols have shown promising results yielding increased tolerance to peanut in allergic children. Adolescence is particularly at risk. METHODS/DESIGN We have designed a randomized, double-blind, placebo-controlled,...