Pepsin reacts stoicheiometrically with the active-site-directed irreversible inhibitor N-diazoacetyl-l-phenylalanine methyl ester, with concomitant loss of all proteolytic and peptidolytic activity. The reagent esterifies a unique aspartic acid residue in pepsin, which is in the sequence:Ile-Val-Asp-Thr-Gly-Thr-Ser

Since 1836, when Theodor Schwann signalized the presence in gastric juice of the proteolytic ferment which he was the first to designate as pepsin, a large number of attempts have been made to isolate it in a pure form to determine its chemical nature and its ultimate physiological properties. In a large majority of these, and, especially, all those made before 1895, except that of Brticke (I),...

IMPORTANCE The role of aspiration-associated extraesophageal reflux disease (AERD) in patients with chronic respiratory symptoms is not well defined. Identifying the frequency of AERD in these patients may provide guidance in their treatment. OBJECTIVE To determine the prevalence of AERD in patients with chronic respiratory symptoms and to assess the utility of pepsin as a new marker for AERD...

The present study was designed to compare collagen synthesized by rabbit lens epithelial cells in culture with rabbit lens capsule collagen. Confluent monolayers of rabbit lens epithelial cells were established. Incorporation of [3H]-proline into glycoproteins secreted into the medium and cell surface components were analyzed in the presence of protease inhibitors. Gel filtration chromatography...

To investigate the mechanism of the anti-peptic action of ecabet sodium (TA-2711) observed in pylorous-ligated rats, effects of this drug on the peptic activity of rat gastric juice, purified hog pepsin and pepsinogen were studied in vitro. After incubation with or without ecabet at acidic pH, the reaction mixture was centrifuged, and the peptic activity of the supernatant was measured. Ecabet ...

BACKGROUND Guidelines recommending head of bed (HOB) elevation greater than 30º to prevent ventilator-associated pneumonia conflict with guidelines to prevent pressure ulcers, which recommend HOB elevation less than 30º. OBJECTIVES To examine the feasibility of 45º HOB elevation and describe and compare the occurrence of reflux, aspiration, and pressure ulcer development at 30º and 45º HOB el...

The effect of pepsin digestion on the allergenicity of raw and thermally processed (boiled and fried) fish muscle extracts of two widely consumed fishes bhetki (Lates calcarifer) and mackerel (Rastrelliger kanagurta) was studied. Sere were collected from 110 patients who were hypersensitive to fish, as evidenced by their clinical history, symptoms and positive skin-prick test results. The vario...

: Pepsin is an aspartate protease that generated from its proenzyme, pepsinogen by autocatalysis initiated a fall in pH below 5. Human gastric juice contains eight isoenzymes of pepsin. The peptides released on conversion to pepsin which there are potentially five, have been shown antimicrobial activity against wide range bacteria including Escherichia coli, Pseudomonas and Staphylococcus also ...

Since 1836, when Theodor Schwann signalized the presence in gastric juice of the proteolytic ferment which he was the first to designate as pepsin, a large number of attempts have been made to isolate it in a pure form to determine its chemical nature and its ultimate physiological properties. In a large majority of these, and, especially, all those made before 1895, except that of Brticke (I),...