Proteolysis of proteins and peptides is involved in the infection cells by enveloped viruses also invasion spread cancer cells. Shutting down broad-specificity proteases, however, problematic because normal functions these proteases will be affected. Herein, nanoparticle receptors were prepared from molecular imprinting for complex biological peptides. Their strong selective binding enabled the...

Abstract Bruce Merrifield (1921‒2006) was an American biochemist who, at the Rockefeller Institute, invented a highly efficient technique for producing peptides and proteins in early 1960s. He awarded Nobel Prize Chemistry 1984. We remember this original, low-profile contributor to chemistry on occasion of one hundredth anniversary his birth.

Polyethylene glycol (PEG) hydrogel microparticles generated via stop-flow lithography can be utilized for efficient microparticle-based cell culture processes because of their high biocompatibility, the molecular diffusion capability in gel structure, and tunability shape size. However, typical functionalization process PEG with cell-adhesion promoters has inevitable limitations, requiring addi...

background: antimicrobial peptides with relative length (2-100 amino acids) and positive charge (pure charge (+9)-(+2)) are amphiphilic that isolated from a wide range of animals. recently, these peptides have been known as a part of innate immune response. nowadays, more than 500 antimicrobial peptides from animals have been reported. objectives: the aim of this study was to evaluate the anti-...

Richard A. Lerner (1938-2021) was both a chemist and an MD. He investigated the structure of peptides proteins, identified sleep-inducing lipid, is best known for his pioneering work on catalytic antibodies. credited with making Scripps Research Institute in La Jolla into world-class center chemistry, biochemistry, biology.

The bioactivity of canary seed peptides (CSP) towards metabolism-regulating enzymes was evaluated. Peptides with angiotensin-converting enzyme (ACE), dipeptidyl peptidase IV (DPP-IV), and pancreatic lipase activity remained stable (p < 0.05) to simulated gastrointestinal digestion (SGD). CSP-SGD were transported efficiently (>10%) through the Caco-2 monolayer, indicating absorption intestinal e...

A new methodology is proposed for the non-target protein analysis of samples from wastewater treatment plants (WWTP). Proteins captured using polymeric (polycaprolactonediol) probes placed along different sites a WWTP were extracted, digested and analyzed by LC-MS/MS. Selection Regions Interest (ROI) raw MS data allowed their storage with most relevant information present in samples. Applicatio...

Abstract Protein engineering has great potential for devising multifunctional recombinant proteins to serve as next-generation protein therapeutics, but it often requires drastic modifications of the parental scaffolds e.g., additional domains at N/C-terminus or replacement a domain by another. A discovery platform system, called RaPID (Random non-standard Peptides Integrated Discovery) enabled...

Abstract Background Although Escherichia coli has been widely used for the expression of exogenous proteins, secretory in this system is still a big obstacle. As one most important secretion pathways, hemolysin A (HlyA) E. can transport substrates directly from cytoplasm to extracellular medium without formation any periplasmic intermediate, making it an ideal candidate development production p...